|
| Status |
Public on Aug 27, 2014 |
| Title |
C5 vs. S5 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
C5
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: primary cancer cell of ovarian serous carcinoma
|
| Growth protocol |
Serum-free DMEM/F12 medium supplemented with 20 ng/ml epidermal growth factor,10 ng/ml basic fibroblast growth factor, 0.4% bovine serum albumin, and 5 µg/mL insulin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
0.2ug of total RNA was labeled using Low Input Quick Amp Labeling Kit, Two-Color (Agilent Technology) as following manufacturer's instructions.
|
| |
|
| Channel 2 |
| Source name |
S5
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Isolated spheroid-forming cell of ovarian serous carcinoma
|
| Growth protocol |
Serum-free DMEM/F12 medium supplemented with 20 ng/ml epidermal growth factor,10 ng/ml basic fibroblast growth factor, 0.4% bovine serum albumin, and 5 µg/mL insulin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
0.2ug of total RNA was labeled using Low Input Quick Amp Labeling Kit, Two-Color (Agilent Technology) as following manufacturer's instructions.
|
| |
|
| |
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| Scan protocol |
Scanned on an Agilent G2565AA scanner.
|
| Description |
Ovarian serous carcinoma_no5_Spheroid-forming cell vs. Corresponding primary cancer cell
|
| Data processing |
Agilent Feature Extraction Software (v 9.3.2.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Aug 26, 2014 |
| Last update date |
Apr 23, 2018 |
| Contact name |
Ah-Young Kwon |
| Organization name |
College of Medicine, CHA University
|
| Department |
Department of Pathology
|
| Street address |
351 Yatap-dong, Bundang-gu
|
| City |
Seongnam-si |
| State/province |
Gyeonggi-do |
| ZIP/Postal code |
463-712 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL17077 |
| Series (1) |
| GSE60765 |
Identification of genes differentailly expressed in cancer stem-like cells of ovarian serous carcinoma and its clinical significance |
|
| Relations |
| Reanalyzed by |
GSE113533 |
