|
| Status |
Public on Jul 23, 2015 |
| Title |
C2-siCon-t=241rep4 |
| Sample type |
SRA |
| |
|
| Source name |
3T3-L1
|
| Organism |
Mus musculus |
| Characteristics |
cell line: 3T3-L1 pre-adipocyte cell line
sirna type: siCon
hours: 24
passage: 12-15
|
| Treatment protocol |
3T3-L1 pre-adipocytes were seeded at 90,000 cells per well in a 12-well plate, followed by transfection with siRNA (control or against 14-3-3ζ). 24 hr after transfection, cells were induced to differentiate with of 172 nM insulin, 500 μM IBMX, and 500 nM dexamethasone in differentiation media containing 25 mM glucose DMEM and 10% fetal bovine serum.
|
| Growth protocol |
3T3-L1 pre-adipocytes were grown in 25mM glucose DMEM, supplemented with 10% newborne calf serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Qiagen Rneasy kit with the on-column Dnase treatment. Samples were stored at -80 C until use.
Libraries were generated using Illumina's Truseq V2 library kit, and each sample was given a unique identifier prior to sequencing on an Illumina HiSeq 2500 (150 bpx2, 20 million paired-end reads)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
Sample 20
|
| Data processing |
Reads were aligned to the mouse genome using Tophat (v.2.0.11)
Differential gene expression was measured using Cufflinks (v.2.2.0)
Analyzed data are expressed in FPKM.
Genome_build: MGSCv37 (Ensembl mouse NCBIM37)
Supplementary_files_format_and_content: Processed data file (.xls) reports FPKM data
|
| |
|
| Submission date |
Aug 26, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Gareth Lim |
| E-mail(s) |
gareth.lim@umontreal.ca
|
| Organization name |
CRCHUM
|
| Street address |
R08.482- 900 rue St Denis
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H2X 0A9 |
| Country |
Canada |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE60745 |
14-3-3ζ controls adipocyte progenitor cell cycle and differentiation via Gli3-dependent p27Kip expression |
|
| Relations |
| BioSample |
SAMN03008162 |
| SRA |
SRX686026 |
