|
| Status |
Public on Apr 27, 2007 |
| Title |
N2 (wt) worms at L4 stage, third replicate |
| Sample type |
RNA |
| |
|
| Source name |
RNA from synchronized at L4 stage C. elegans population
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
L4 stage N2 hermaphrodites
|
| Biomaterial provider |
David Fay
|
| Growth protocol |
Worms were grown on NGM plates spotted with OP50 at 20 oC.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with phenol, cleaned with phenol:chlorophorm:isoamyl alcohol (25:24:1), and then with chlorophorm:isoamyl alcohol (24:1). Total RNA was precipitated overnight at 4 oC with 4M lithium acetate and DNAseI treated before purification on RNEasy minicolumns from Qiagen.
|
| Label |
biotin
|
| Label protocol |
2.5 ug total RNA were used for cDNA synthesis. cDNA synthesis, cRNA synthesis, fragmentation and labeling were performed according to Affymetrix protocol.
|
| |
|
| Hybridization protocol |
according to Affymetrix protocol
|
| Scan protocol |
according to Affymetrix protocol
|
| Description |
L4 stage N2 hermaphrodites
|
| Data processing |
expression values were generated using RMA
|
| |
|
| Submission date |
Nov 28, 2006 |
| Last update date |
Feb 21, 2007 |
| Contact name |
David Fay |
| E-mail(s) |
davidfay@uwyo.edu
|
| Organization name |
University of Wyoming
|
| Department |
MOLB
|
| Street address |
1000 E. University Dept. 3944
|
| City |
Laramie |
| State/province |
WY |
| ZIP/Postal code |
82071 |
| Country |
USA |
| |
|
| Platform ID |
GPL200 |
| Series (1) |
| GSE6547 |
Developmental transcriptome profiling of the C. elegans pocket protein ortholog, lin-35 |
|
