|
| Status |
Public on Sep 11, 2014 |
| Title |
BXD101 Liver, Chow Diet |
| Sample type |
RNA |
| |
|
| Source name |
Mouse liver
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver
genotype: BXD101
|
| Treatment protocol |
Mice were fed ad libitum chow diet (Harlan 2018) or high fat diet (Harlan 06414) throughout their lifetime. For high fat cohorts, the diet started at 8 weeks of age until sacrifice at 29 weeks. All animals were fasted overnight (6pm to 9am) before sacrifice.
|
| Growth protocol |
Mice are housed 5 per cage in IVC GM500 cages (Tecniplast, 500 cm2) according to the Swiss cantonal rules.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed on all individuals -- approximately 5 per cohort, for roughly 400 animals in all. Sample concentrations were then measured by Nanodrop, and then all individuals from a cohort were evenly pooled together. These cohort pools were then cleaned up with a Qiagen RNEasy kit prior to preparation for microarray.
|
| Label |
biotin
|
| Label protocol |
100ng of total RNA was amplified using Ambion® WT Expression Kit from life technologies (Part Number 4411974) according to manufactures protocols, then 5500ng cDNA was fragmented and labeled using Affymetrix WT terminal labeling kit (part number 900671) according to manufactures protocols.
|
| |
|
| Hybridization protocol |
Labeled cDNA was hybridized according to Affymetrix protocols. Approximately 16hr incubation and then washed and stained according to Affymetrix protocols on Affymetrix 450 Fluidics Station.
|
| Scan protocol |
Arrays were scanned on Affymetrix GSC3000 7G Scanner
|
| Description |
Gene expression data
|
| Data processing |
Samples were analyzed using Affymetrix Expression Console.
|
| |
|
| Submission date |
Aug 06, 2014 |
| Last update date |
Sep 11, 2014 |
| Contact name |
Johan Auwerx |
| E-mail(s) |
admin.auwerx@epfl.ch
|
| Phone |
+41 216939522
|
| Organization name |
École Polytechnique Fédérale de Lausanne
|
| Department |
SV IBI NCEM
|
| Lab |
Integrative and Systems Physiology
|
| Street address |
Station 15
|
| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE60149 |
Global hepatic transcript data from fasted male BXD strains on chow or high fat diet |
|
