GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM1421016 Query DataSets for GSM1421016
Status Public on Jan 01, 2015
Title Input DNA rep1
Sample type SRA
Source name Dendritic cells
Organism Homo sapiens
Characteristics cell type: Monocyte-derived denditric cells
chip antibody: none
Growth protocol monocytes were cultured in RPMI 10% FCS supplemented with 2-mercaptoethanol (50 μM), GM-CSF (600 U/ml), and IL-4 (750 U/ml)
Extracted molecule genomic DNA
Extraction protocol Chromatine was isolated, fragmented by sonication and immunoprecipitate it with anti PU1 antibody
Libraries were prepared according to Illumina's instructions using the Truseq ChIP-seq protocol.
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2500
Data processing Basecalls performed using CASAVA version 1.8.2
Reads were mapped to the reference genome using bowtie 0.12.7 with -m 1
Peaks were called using macs2 with -p 0.1
Genome_build: GRCh37.71
Submission date Jun 26, 2014
Last update date May 15, 2019
Contact name Sylvain LEMEILLE
Organization name University of Geneva Medical School
Department Department of Pathology and Immunology
Street address 1 rue Michel-Servet
City Geneva
ZIP/Postal code 1211
Country Switzerland
Platform ID GPL16791
Series (1)
GSE58864 Extensive remodeling of DC function by rapid maturation-induced epigenetic gene silencing
BioSample SAMN02887302
SRA SRX627427
Named Annotation

Supplementary file Size Download File type/resource 224.6 Mb (ftp)(http) BW
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap