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Series GSE58864 Query DataSets for GSE58864
Status Public on Jan 01, 2015
Title Extensive remodeling of DC function by rapid maturation-induced epigenetic gene silencing
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Dendritic-cell (DC) maturation involves substantial remodeling of their gene-expression program. Most research has focused on inducible gene-expression networks promoting the acquisition of new functions, such as cytokine production and enhanced T-cell-stimulatory capacity. In contrast, mechanisms that modulate DC-function by inducing gene silencing remain poorly understood. Here we describe a novel primary epigenetic-silencing response that makes major contributions to the DC-maturation process. The repressed genes function in pivotal processes - including antigen-presentation, extracellular-signal detection, signal-transduction and lipid-mediator biosynthesis - underscoring the central contribution of the silencing mechanism to rapid reshaping of DC-function. Interestingly, promoters of the repressed genes exhibit a surprisingly high frequency of PU.1-occupied sites, suggesting a novel role for this transcription factor in marking genes poised for inducible repression
Overall design Analysis of PU.1 binding sites in mo-DC
Contributor(s) Seguín-Estévez Q, Dunand-Sauthier I, Lemeille S, Iseli C, Ibberson M, Ioannidis V, Barras E, Geinoz A, Schmid CD
Citation(s) 25104025
Submission date Jun 26, 2014
Last update date May 15, 2019
Contact name Sylvain LEMEILLE
Organization name University of Geneva Medical School
Department Department of Pathology and Immunology
Street address 1 rue Michel-Servet
City Geneva
ZIP/Postal code 1211
Country Switzerland
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (4)
GSM1421016 Input DNA rep1
GSM1421017 Pu.1_ChIPseq rep1
GSM1421018 Input DNA rep2
BioProject PRJNA253735
SRA SRP043611

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Supplementary file Size Download File type/resource
GSE58864_RAW.tar 1.2 Gb (http)(custom) TAR (of BED, BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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