|
| Status |
Public on Jul 31, 2014 |
| Title |
genomic DNA from bone marrow CD34+ cells 5729917060_RO5CO2 |
| Sample type |
genomic |
| |
|
| Source name |
normal bone marrow progenitor cells
|
| Organism |
Homo sapiens |
| Characteristics |
sample id: CD34-3
gender: Male
age at diagnosis in years: n.a.
batch: batch1
cell type: normal bone marrow progenitor cells
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA and RNA was extracted by TRIzol ( Invitrogen) or ALLPrep ( QIAGEN ) according to the standard instructions. Sample quanlity were tested by Genomic DNA ScreenTape.
|
| Label |
Cy5 and Cy35
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
normal bone marrow progenitor cells
|
| Data processing |
The raw data was processed by GenomeStudio (QIAGEN) by standard protocol.
|
| |
|
| Submission date |
Jun 13, 2014 |
| Last update date |
Jul 31, 2014 |
| Contact name |
Ying Qu |
| Organization name |
Karolinska Institute
|
| Department |
Department of Medicine, Huddinge
|
| Lab |
Center of Hematology and Regenerative Medicine (HERM)
|
| Street address |
Hälsovagen 7
|
| City |
Stockholm |
| State/province |
Stockholm |
| ZIP/Postal code |
14157 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE58477 |
Differential methylation in CN-AML preferentially targets non-CGI regions and is dictated by DNMT3A mutational status and associated with predominant hypomethylation of HOX genes |
|
