|
| Status |
Public on Jul 30, 2014 |
| Title |
LCL_untreated [NM03509] |
| Sample type |
RNA |
| |
|
| Source name |
LCL_untreated
|
| Organism |
Homo sapiens |
| Characteristics |
in vitro treated ifnb: NO
gender: Female
ms subtype: Relapsing Remitting
treatment status at phlebotomy: Treated
age: 33
ethnicity (jewish/arab): J
|
| Treatment protocol |
LCLs (1.4 million cells/ml) were incubated with 100 units/ml recombinant human IFN-β1a (InterferonSource) for 4 hours. Untreated cells were cultured in parallel and under identical culture conditions for comparison.
|
| Growth protocol |
LCLs were cultured in RPMI-1640 supplemented with 10% FBS, 2 mM L-glutamine, penicillin (100 U/ml), streptomycin (100μg/ml), and nystatin (12.5U/ml, Biological Industries).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from LCL cultures using the Roche RNA isolation kit. All RNA samples were checked for quality using Experion (Bio-Rad).
|
| Label |
biotin
|
| Label protocol |
Illumina Protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
Genome Studio software-detected intensity for values in sample columns and detection P values in the non-normalized matrix sheet. The data was log2 transformed and batch normalized using JMP genomics batch normalization application.
|
| |
|
| Submission date |
Jun 05, 2014 |
| Last update date |
Jul 30, 2014 |
| Contact name |
Tamar Paperna |
| E-mail(s) |
paperna@tx.technion.ac.il
|
| Phone |
972-4-8295261
|
| Organization name |
Technion
|
| Department |
Faculty of Medicine
|
| Street address |
Efron St. 1
|
| City |
Haifa |
| ZIP/Postal code |
31096 |
| Country |
Israel |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE58240 |
Gene expression profiling of the response to interferon beta in EBV-transformed and primary B cells of patients with multiple sclerosis |
|
