|
| Status |
Public on Jul 30, 2014 |
| Title |
Gene expression profiling of the response to interferon beta in EBV-transformed and primary B cells of patients with multiple sclerosis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To identify gene expression changes and pathways induced by interferon-β (IFN-β) in B cells, we studied the in vitro response of EBV-transformed B cells (lymphoblast cell lines-LCLs). LCLs were derived from an MS patient repository. Whole genome expression analysis identified 115 genes that were more than two-fold differentially up-regulated following IFN-β exposure, with over 50 novel IFN-β response genes. Pathways analysis demonstrated that IFN-β affected LCLs in a similar manner to other cell types by activating known IFN-β canonical pathways. Additionally, IFN-β increased the expression of innate immune response genes, while down-regulating many B cell receptor pathway genes and genes involved in adaptive immune responses. Several of the novel response genes were tested and validated as IFN-β response genes in human primary B cells.
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| |
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| Overall design |
Total RNA from LCL samples treated for four hours with interferon beta (100u/ml) was compared to RNA from untreated LCLs (paired analysis). LCL samples were from patients with MS.
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| |
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| Contributor(s) |
Khsheibun R, Paperna T, Avidan N, Miller A |
| Citation(s) |
25025430 |
| |
| Submission date |
Jun 05, 2014 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Tamar Paperna |
| E-mail(s) |
paperna@tx.technion.ac.il
|
| Phone |
972-4-8295261
|
| Organization name |
Technion
|
| Department |
Faculty of Medicine
|
| Street address |
Efron St. 1
|
| City |
Haifa |
| ZIP/Postal code |
31096 |
| Country |
Israel |
| |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
|
| Samples (32)
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|
| Relations |
| BioProject |
PRJNA251683 |
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