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Sample GSM1394964 Query DataSets for GSM1394964
Status Public on Jan 01, 2018
Title mESC [re-analysis]
Sample type SRA
Source name mouse embryonic stem cells(mESCs)
Organism Mus musculus
Characteristics cell type: OG2 transgenetic mouse embryonic stem cells(OG2ES)
time: P5
tag: 1/2 129SVJ+3/8 C57B6+1/8 CBA
Extracted molecule total RNA
Extraction protocol Total RNA was extracted by Trizol-based protocol.
The libraries were prepared according to Illumina TruSeq® RNA sample preparation Kit.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina MiSeq
Description WT OG2ES
Data processing Illumina Casava1.8 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence.
Transcripts per million(TPM) were calculated using RSEM(1.1.21) with parameters(-p 8 --estimated-rspd --no-bam-output).
Genome_build: mm9
Submission date May 20, 2014
Last update date May 15, 2019
Contact name Lihui Lin
Phone 020-32015291
Organization name Guangzhou Institutes Of Biomedicine and Health Chinese Academy Of Science
Department Stem cell
Lab Pei Duanqing
Street address No.190, Kaiyuan Street
City Guangzhou
State/province Guangdong
ZIP/Postal code GD20
Country China
Platform ID GPL16417
Series (1)
GSE57546 A Direct and Accelerated Platform for Analyzing Reprogramming by Defined factors
Reanalysis of GSM1229023
BioSample SAMN02795956
SRA SRX547505

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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