|
Status |
Public on Jan 01, 2018 |
Title |
mESC [re-analysis] |
Sample type |
SRA |
|
|
Source name |
mouse embryonic stem cells(mESCs)
|
Organism |
Mus musculus |
Characteristics |
cell type: OG2 transgenetic mouse embryonic stem cells(OG2ES) time: P5 tag: 1/2 129SVJ+3/8 C57B6+1/8 CBA
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted by Trizol-based protocol. The libraries were prepared according to Illumina TruSeq® RNA sample preparation Kit.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina MiSeq |
|
|
Description |
WT OG2ES GSM1229023
|
Data processing |
Illumina Casava1.8 software used for basecalling. Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence. Transcripts per million(TPM) were calculated using RSEM(1.1.21) with parameters(-p 8 --estimated-rspd --no-bam-output). Genome_build: mm9
|
|
|
Submission date |
May 20, 2014 |
Last update date |
May 15, 2019 |
Contact name |
Lihui Lin |
E-mail(s) |
lin_lihui@gibh.ac.cn
|
Phone |
020-32015291
|
Organization name |
Guangzhou Institutes Of Biomedicine and Health Chinese Academy Of Science
|
Department |
Stem cell
|
Lab |
Pei Duanqing
|
Street address |
No.190, Kaiyuan Street
|
City |
Guangzhou |
State/province |
Guangdong |
ZIP/Postal code |
GD20 |
Country |
China |
|
|
Platform ID |
GPL16417 |
Series (1) |
GSE57546 |
A Direct and Accelerated Platform for Analyzing Reprogramming by Defined factors |
|
Relations |
Reanalysis of |
GSM1229023 |
BioSample |
SAMN02795956 |
SRA |
SRX547505 |