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Sample GSM1229023 Query DataSets for GSM1229023
Status Public on Jun 10, 2015
Title mESC
Sample type SRA
Source name mouse embryonic stem cells(mESCs)
Organism Mus musculus
Characteristics cell type: OG2 transgenetic mouse embryonic stem cells(OG2ES)
passages: P5
strain: 1/2 129SVJ+3/8 C57B6+1/8 CBA
genotype/variation: WT OG2ES
Extracted molecule total RNA
Extraction protocol Total RNA was extracted by Trizol-based protocol.
The libraries were prepared according to Illumina TruSeq® RNA sample preparation Kit.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina MiSeq
Description WT OG2ES
Data processing Illumina Casava1.8 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence.
Transcripts per million(TPM) were calculated using RSEM(1.1.21) with parameters(-p 8 --estimated-rspd --no-bam-output).
Genome_build: mm9
Supplementary_files_format_and_content: TXT files including tau values for each RNA-seq samples, and TPM values are generated for all the samples.
Submission date Sep 11, 2013
Last update date May 15, 2019
Contact name Lihui Lin
Phone 020-32015291
Organization name Guangzhou Institutes Of Biomedicine and Health Chinese Academy Of Science
Department Stem cell
Lab Pei Duanqing
Street address No.190, Kaiyuan Street
City Guangzhou
State/province Guangdong
ZIP/Postal code GD20
Country China
Platform ID GPL16417
Series (1)
GSE50776 c-Jun impedes somatic cell reprogramming by activating mesenchymal core regulators and suppressing pluripotent ones
Reanalyzed by GSM1394964
BioSample SAMN02354058
SRA SRX348623

Supplementary file Size Download File type/resource
GSM1229023_mESC.genes.results.txt.gz 355.4 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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