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Status
Public on Oct 02, 2014
Title
patient BH014, low responder, male, day2, hour 4, baseline
Sample type
RNA
Source name
blood sample from patient BH014, low responder, male, day2, hour 4, baseline
Organism
Homo sapiens
Characteristics
patient: BH014 responder: low Sex: male day: 2 hour: 4 biological group: baseline
Extracted molecule
total RNA
Extraction protocol
PAXgene protocol was used to extract total RNA
Label
biotin
Label protocol
RT/IVT protocol and reagents provided by Affymetrix
Hybridization protocol
Following fragmentation, 10 ug of cDNA was hybridized for 18 hr at 45C. GeneChips were washed and stained in the Affymetrix
Scan protocol
Affymetrix GeneChip Scanner 3000
Description
blood sample from patient BH014, lowresponder, male, day2, hour 4, baseline
Data processing
Data were processed using R/affy software. In brief, the RMA method adjusts the background of perfect match (PM) probes, applies a quantile normalization of the corrected PM values, and calculates final expression measures with the Tukey median polish algorithm.
Submission date
Apr 21, 2014
Last update date
Oct 03, 2014
Contact name
Keith Shockley
Organization name
NIEHS
Street address
111 TW Alexander Drive
City
RTP
State/province
NC
ZIP/Postal code
27709
Country
USA
Platform ID
GPL10379
Series (1)
GSE56931
Blood gene expression reveals reduced circadian rhythmicity in individuals resistant to sleep deprivation
Data table header descriptions
ID_REF
VALUE
RMA expression measures from R/affy package
Data table
ID_REF
VALUE
100121619_TGI_at
6.348546
100121620_TGI_at
7.936631
100121621_TGI_at
6.130995
100121622_TGI_at
7.433204
100121623_TGI_at
8.982936
100121624_TGI_at
7.258876
100121625_TGI_at
8.231812
100121626_TGI_at
3.764584
100121627_TGI_at
5.28225
100121628_TGI_at
4.381063
100121629_TGI_at
9.685613
100121630_TGI_at
5.700455
100121631_TGI_at
4.245872
100121632_TGI_at
7.548666
100121633_TGI_at
8.962645
100121634_TGI_at
3.715122
100121635_TGI_at
8.341404
100121636_TGI_at
5.508213
100121637_TGI_at
6.516296
100121638_TGI_at
5.677688
Total number of rows: 52378 Table truncated, full table size 1335 Kbytes .Supplementary file
Size
Download
File type/resource
GSM1371645__52070900797038031010407111021306_1_1.CEL.gz
5.4 Mb
(ftp) (http)
CEL
Processed data included within Sample table