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Status
Public on Oct 02, 2014
Title
patient BH005, high responder, female, day2, hour 4, baseline
Sample type
RNA
Source name
blood sample from patient BH005, high responder, female, day2, hour 4, baseline
Organism
Homo sapiens
Characteristics
patient: BH005 responder: high Sex: female day: 2 hour: 4 biological group: baseline
Extracted molecule
total RNA
Extraction protocol
PAXgene protocol was used to extract total RNA
Label
biotin
Label protocol
RT/IVT protocol and reagents provided by Affymetrix
Hybridization protocol
Following fragmentation, 10 ug of cDNA was hybridized for 18 hr at 45C. GeneChips were washed and stained in the Affymetrix
Scan protocol
Affymetrix GeneChip Scanner 3000
Description
blood sample from patient BH005, highresponder, female, day2, hour 4, baseline
Data processing
Data were processed using R/affy software. In brief, the RMA method adjusts the background of perfect match (PM) probes, applies a quantile normalization of the corrected PM values, and calculates final expression measures with the Tukey median polish algorithm.
Submission date
Apr 21, 2014
Last update date
Oct 03, 2014
Contact name
Keith Shockley
Organization name
NIEHS
Street address
111 TW Alexander Drive
City
RTP
State/province
NC
ZIP/Postal code
27709
Country
USA
Platform ID
GPL10379
Series (1)
GSE56931
Blood gene expression reveals reduced circadian rhythmicity in individuals resistant to sleep deprivation
Data table header descriptions
ID_REF
VALUE
RMA expression measures from R/affy package
Data table
ID_REF
VALUE
100121619_TGI_at
5.886518
100121620_TGI_at
7.342238
100121621_TGI_at
7.590777
100121622_TGI_at
8.348642
100121623_TGI_at
8.790847
100121624_TGI_at
7.561992
100121625_TGI_at
8.743649
100121626_TGI_at
4.046655
100121627_TGI_at
4.803702
100121628_TGI_at
3.920516
100121629_TGI_at
10.078859
100121630_TGI_at
6.636374
100121631_TGI_at
4.11065
100121632_TGI_at
7.960692
100121633_TGI_at
8.992183
100121634_TGI_at
3.77131
100121635_TGI_at
8.574608
100121636_TGI_at
5.341685
100121637_TGI_at
6.829808
100121638_TGI_at
5.598292
Total number of rows: 52378 Table truncated, full table size 1335 Kbytes .Supplementary file
Size
Download
File type/resource
GSM1371588__52070900796934030810407096011647_1_1.CEL.gz
5.2 Mb
(ftp) (http)
CEL
Processed data included within Sample table