|
| Status |
Public on Mar 22, 2014 |
| Title |
ZHBTc4-TSC biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
ZHBTc4 TS cells, +Tet
|
| Organism |
Mus musculus |
| Characteristics |
strain: 129/Ola
cell line: ZHBTc4
treatment: Tet
|
| Treatment protocol |
Tet-inducible Oct3/4 knockout ES cells were cultured in TS cell medium supplemented with 1 ug/ml Tet for 4 days on MEF feeders.
|
| Growth protocol |
ES cells were maintained in GMEM supplemented with 10% FBS and 1,000 U/ml LIF on MEF feeders. TS cells were maintained in GMEM/10% FBS supplemented with 50 ng/ml human FGF4, 2 ug/ml heparin, 10 ng/ml human Activin A and 2 mM L-glutamine on MEF feeders.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using QuickGene RNA cultured cell HC kit S (Fuji Film).
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized, stained, and washed according to the Expression Analysis Technical Manual (Affymetrix).
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 7G
|
| Description |
On MEF feeders, with FGF4/heparin/Activin A
|
| Data processing |
The expression values were summarized and normalized by the robust multiarray analysis and quantile normalization method using by Expression Console(tm) software (Affymetrix).
MoEx-1_0-st-v1.r2.pgf
MoEx-1_0-st-v1.r2.dt1.mod.mm9.mps
|
| |
|
| Submission date |
Mar 21, 2014 |
| Last update date |
Mar 22, 2014 |
| Contact name |
Itoshi NIKAIDO |
| E-mail(s) |
itoshi.nikaido@riken.jp
|
| Organization name |
RIKEN
|
| Department |
Center for Biosystems Dynamics Research
|
| Lab |
Laboratory for Bioinformatics Research
|
| Street address |
2-1 Hirosawa
|
| City |
Wako |
| State/province |
Saitama |
| ZIP/Postal code |
351-0198 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6096 |
| Series (2) |
| GSE28452 |
Induction of trophoblast stem cells from embryonic stem cells by forced repression of Oct3/4. |
| GSE28455 |
Sox2-regulatory networks in embryonic and trophoblast stem cells |
|
