|
| Status |
Public on Mar 05, 2017 |
| Title |
ES2_gene expression |
| Sample type |
RNA |
| |
|
| Source name |
ovarian cancer cell line ES2
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: ovarian cancer cell line
|
| Growth protocol |
Cells were grown in complete RPMI medium 1640 supplemented with 10% fetal bovine serum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen's column-based RNA extraction protocol.
|
| Label |
biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
Human Gene 1.0 ST microarrays were hybridized with 10 µg cDNA and processed according to the manufacturer's protocol (Affymetrix).
|
| Scan protocol |
As per manufacturer (Affymetrix)
|
| Description |
Hybridized to Human Gene 1.0 ST array
|
| Data processing |
The data from gene expression microarrays were analyzed using Partek Genomics Suite 6.6 software (Partek).
|
| |
|
| Submission date |
Mar 05, 2014 |
| Last update date |
Mar 05, 2017 |
| Contact name |
Ichiro Nakachi |
| E-mail(s) |
ichiro.nakachi@ucdenver.edu
|
| Phone |
303-724-6053
|
| Organization name |
University of Colorado Denver
|
| Department |
Division of Pulmonary Sciences and Critical Care Medicine, Department of Medicine
|
| Lab |
Mark Geraci's Lab
|
| Street address |
12700 East 19th Avenue, RC2 9th floor
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE55628 |
Differentially expressed genes and chromosomal alterations in ovarian cancer cell lines sensitive or resistant to a Src-inihibitor, saracatinib |
|
