|
| Status |
Public on Feb 20, 2015 |
| Title |
E84CVP-1 |
| Sample type |
SRA |
| |
|
| Source name |
Cardiac progenitor cell-derived cardiac myocytes
|
| Organism |
Homo sapiens |
| Characteristics |
developmental state: day 14
cell line: RUES2 hESCs
cell type: Cardiac progenitor cell-derived cardiac myocytes
|
| Growth protocol |
Monolayer directed differentiation using Activin A and BMP4 for cardiac progenitor differentiation to cardiomyocytes. Monolayer directed differentiation with Activin A and BMP4 following by treatment with VEGF on day 2 for derivation of hemogenic endothelial cells. Hemogenic endothelial cells were then treated with 2 uM XAV to direct cardiac lineage development
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA-isolation
pA enrichment
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
isoform_fpkm.tsv, gene_fpkm.tsv
|
| Data processing |
Reads were mapped to hg19 using tophat 1.3.3.
Genome_build: hg19
Supplementary_files_format_and_content: Tab-delimited files with FPKM estimates at the gene (gene_fpkm.tsv) and isoform (isoform_fpkm.tsv) level, generated using Cufflinks.
|
| |
|
| Submission date |
Feb 22, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Chuck Murry |
| E-mail(s) |
murry@u.washington.edu
|
| Phone |
(206) 616-8684
|
| Organization name |
University of Washington
|
| Department |
Institute for Stem Cell and Regenerative Medicine, Center for Cardiovascular Biology
|
| Street address |
850 Republican St., Box 358050
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE55275 |
Activin A and BMP4 Modulation of Wnt/β-catenin Signaling Directs Fate Specification of Mesoderm Derivatives from Human Embryonic Stem Cells |
|
| Relations |
| BioSample |
SAMN02649625 |
| SRA |
SRX475019 |
