|
| Status |
Public on Apr 22, 2008 |
| Title |
KOPTK1 CompE 24h rep2 |
| Sample type |
RNA |
| |
|
| Source name |
KOPTK1 human T-cell lymphoblastic cell line gamma-secretase inhibitor (CompE 500nM) treatment 24h
|
| Organism |
Homo sapiens |
| Characteristics |
T-cell acute lymphoblastic leukemia cell line
|
| Biomaterial provider |
T-cell acute lymphoblastic leukemia cell line kindly provided by Dr. A Thomas Look at Dana-Farber Cancer Institute, Boston, Massachusetts, US.
|
| Treatment protocol |
Compound E (CompE) [ (2S)-2-{[ (3,5-Difluorophenyl)acetyl]amino}-N-[(3S)-1-methyl-2-oxo-5-phenyl-2,3-dihydro-1H-1,4-benzodiazepin-3-yl] propanamide] (Alexis Biochemicals) a cell permeable, potent, selective, non-transition state and non-competitive inhibitor of gamma-secretase treatment at 500nM in DMSO for 24h.
|
| Growth protocol |
Cells were cultured in RPMI1640 media supplemented with 10% fetal bovine serum plus antibiotics (pen/strep) in a humidified incubator with 5% CO2 atmosphere at 37C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with RNAqueous minikit (Ambion) following the manufacturer’s instructions
|
| Label |
biotin
|
| Label protocol |
Approx. 10micrograms of total RNA was processed to produce biotinylated cRNA targets
|
| |
|
| Hybridization protocol |
Standard Affymetrix procedure
|
| Scan protocol |
Standard Affymetrix procedure
|
| Description |
Standard Affymetrix procedure
|
| Data processing |
Affymetrix Microarray Suite Version 5 followed by Dchip data normalization for adjustment of interarray intensity differences and calculation of model based expression (VALUE).
|
| |
|
| Submission date |
Aug 31, 2006 |
| Last update date |
Apr 22, 2008 |
| Contact name |
Adolfo A Ferrando |
| E-mail(s) |
af2196@columbia.edu
|
| Phone |
212-851-4611
|
| Organization name |
Columbia University
|
| Department |
Institute for Cancer Genetics
|
| Street address |
1130 St Nicholas Ave Irving Cancer Research Center 5-505A
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE5716 |
Gene expression analysis of T-ALL cell lines treated with gamma-secretase inhibitor |
| GSE5827 |
NOTCH1 directly regulates c-MYC and activates a feed-forward-loop transcriptional network promoting leukemic cell growth |
|
