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Series GSE5716 Query DataSets for GSE5716
Status Public on Aug 20, 2008
Title Gene expression analysis of T-ALL cell lines treated with gamma-secretase inhibitor
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Gain-of-function mutations in NOTCH1 are common in T-cell lymphoblastic leukemias making this receptor a promising target for drugs such as gamma-secretase inhibitors (GSI), which block a proteolytic cleavage required for NOTCH1 activation. However, the enthusiasm for these therapies has been tempered by tumor resistance and the paucity of information on the oncogenic programs regulated by oncogenic NOTCH1. Analysis of gene expression in GSI-responsive and GSI-resistant cell lines treated with Compound E identifies differential resopnses to GSI.
Keywords: Drug response
 
Overall design Samples for microarray analysis were prepared and hybridized in Affymetrix Human U133 Plus 2.0 arrays according to the manufacturer’s instructions and as previously described. RNA was extracted from duplicate cultures of GSI-sensitive (ALL-SIL, CUTLL1, DND41, HPB-ALL, KOPTK1) and GSI-resistant (CCRF-CEM, MOLT3, P12 ICHIKAWA, PF382 and RPMI8402) T-ALL cell lines treated for 24 h with vehicle (DMSO) or 500 nM CompE. Interarray intensity differences were normalized with Dchip.
 
Contributor(s) Palomero T, Sulis M, Cortina M, Real PJ, Barnes K, Ciofani M, Caparros E, Buteau J, Brown K, Perkins SL, Bhagat G, Mishra A, Basso G, Parsons R, Zúñiga-Pflücker J, Dominguez M, Ferrando AA
Citation(s) 17873882
Submission date Sep 01, 2006
Last update date Mar 25, 2019
Contact name Adolfo A Ferrando
E-mail(s) af2196@columbia.edu
Phone 212-851-4611
Organization name Columbia University
Department Institute for Cancer Genetics
Street address 1130 St Nicholas Ave Irving Cancer Research Center 5-505A
City New York
State/province NY
ZIP/Postal code 10032
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (40)
GSM132932 ALLSIL CompE 24h rep1
GSM132933 ALLSIL CompE 24h rep2
GSM132934 CCRF-CEM CompE 24h rep1
Relations
BioProject PRJNA97007

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Supplementary file Size Download File type/resource
GSE5716_RAW.tar 117.0 Mb (http)(custom) TAR (of CEL)

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