|
| Status |
Public on Jan 18, 2014 |
| Title |
Intratumoral heterogeneity of prostate cancers, patient 3, biopsy 2 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
patient 3_primary prostate cancer
|
| Organism |
Homo sapiens |
| Characteristics |
patient id: Patient 3
age (diagnosis): 68
gleason score: 4+3
tnm stage: pT3b
psa (ng/ml): 12.27
tissue: Primary prostate cancer
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using phenol-chloroform extraction method. The purity and quantity of the DNA was estimated using Nanodrop spectrophotometer.
|
| Label |
Cy5
|
| Label protocol |
DNA labelling and hybridization was performed according to the manufacturer's instruction
|
| |
|
| Channel 2 |
| Source name |
patient 3_adjacent normal tissue
|
| Organism |
Homo sapiens |
| Characteristics |
patient id: Patient 3
age (diagnosis): 68
gleason score: 4+3
tnm stage: pT3b
psa (ng/ml): 12.27
tissue: matched normal (adjacent)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using phenol-chloroform extraction method. The purity and quantity of the DNA was estimated using Nanodrop spectrophotometer.
|
| Label |
Cy3
|
| Label protocol |
DNA labelling and hybridization was performed according to the manufacturer's instruction
|
| |
|
| |
| Hybridization protocol |
standard Agilent protocol
|
| Scan protocol |
Scanned on an Agilent Technologies Scanner G2505C
Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1).
|
| Description |
PS352-2
|
| Data processing |
Signal intensity extraction, and normalization were performed using the Agilent Feature Extraction software 10.7.3.1. Linear normalization method with default values was used in signal intensity extraction procedure.
|
| |
|
| Submission date |
Jan 17, 2014 |
| Last update date |
Jan 18, 2014 |
| Contact name |
Tae-Min Kim |
| E-mail(s) |
tmkim@catholic.ac.kr
|
| Phone |
82-2-2258-7753
|
| Organization name |
The Catholic University of Korea, College of Medicine
|
| Department |
Dept of Medical Informatics
|
| Street address |
Seocho-Gu, Banpo-Dong
|
| City |
Seoul |
| ZIP/Postal code |
137-701 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL10123 |
| Series (1) |
| GSE54185 |
Intratumoral heterogeneity of prostate cancers |
|
