|
| Status |
Public on Jan 14, 2014 |
| Title |
Normal_Control_Pirc_rat19 |
| Sample type |
RNA |
| |
|
| Source name |
normal distal colon
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: (ACIxF344)F1-Pirc
genotype: Pirc/+
treatment: Control
age: 97 days
|
| Treatment protocol |
Indicated animals given 4% DSS (500,000 MW) in drinking water from 40-47 and 54-61 days of age. Sacrificed and dissected at 97 days, samples homogenized in RLTplus and stored at -80C
|
| Growth protocol |
Ad lib feeding and drinking. 12hours light:12hours dark.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Qiashredder columns followed by Qiagen Allprep DNA/RNA Extraction Kit with optional on-the-column Dnase treatment
|
| Label |
Cy3
|
| Label protocol |
Total RNA was amplified and labeled according to manufacturer instructions. Agilent One-Color Microarray-Based Gene Expression Analysis Agilent low RNA input linear amplification kit, PLUS
|
| |
|
| Hybridization protocol |
Agilent One-Color Microarray-Based Gene Expression Analysis 17hrs 65 deg celcius Surehyb chamber
|
| Scan protocol |
Agilent Technologies Scanner G2505B US22502567 5um 16bit
|
| Data processing |
Agilent Feature extraction versions 9.1 and 9.5 for background subtraction - localbackground and normalization Linear LOWESS. No quantile normalization was deemed necessary by Partek software.
|
| |
|
| Submission date |
Jan 12, 2014 |
| Last update date |
Jan 14, 2014 |
| Contact name |
Amy Irving |
| Organization name |
University of Wisconsin-Madison
|
| Department |
McArdle Laboratory for Cancer Research
|
| Lab |
William Dove
|
| Street address |
1400 University Avenue
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platform ID |
GPL14797 |
| Series (2) |
| GSE54005 |
Dextran sodium sulfate treatment of wild type and Pirc rats alters colonic normal tissue gene expression |
| GSE54036 |
Effects of dextran sodium sulfate on normal colonic tissue and tumors in wild type and Apc-Pirc/+ rats. |
|
