|
| Status |
Public on Jan 09, 2014 |
| Title |
shCtrl_3 |
| Sample type |
RNA |
| |
|
| Source name |
KYSE150
|
| Organism |
Homo sapiens |
| Characteristics |
shRNA: Scrambled
|
| Treatment protocol |
KYSE150 cells were transfected with the indicated pRetrosuper shRNA constructs using Fugene 6 (Roche Diagnostics) according to manufacturer's instructions and selected 24h after transfection with 2 μg/ml puromycin for 72 h prior to harvesting.
|
| Growth protocol |
KYSE150 cells were cultured in D-MEM/F-12 1:1 (Invitrogen) supplemented with 10% FBS (Hyclone).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Samples were processed with the Whole Transcript Assay Kit as recommended by the manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
Affymetrix standard procedure
|
| Scan protocol |
Affymetrix standard procedure
|
| Data processing |
R/Bioconductor. 'affy' package. Raw data processed using the 'rma' function.
|
| |
|
| Submission date |
Jan 08, 2014 |
| Last update date |
Jan 09, 2014 |
| Contact name |
Marianne Terndrup Pedersen |
| E-mail(s) |
marianne.pedersen@bric.ku.dk
|
| Organization name |
BRIC
|
| Department |
University of Copenhagen
|
| Lab |
Kim B. Jensen
|
| Street address |
Ole Maaloes Vej 5
|
| City |
Copenhagen N |
| ZIP/Postal code |
2200 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL571 |
| Series (2) |
| GSE28332 |
The Demethylase JMJD2C/KDM4C Localizes to H3K4me3 Positive Transcription Start Sites |
| GSE53892 |
The Demethylase JMJD2C/KDM4C Localizes to H3K4me3 Positive Transcription Start Sites (Affymetrix microarray analysis) |
|
