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Sample GSM1296888 Query DataSets for GSM1296888
Status Public on Dec 24, 2013
Title III-7_Affected_Skin
Sample type RNA
Source name Skin biopsy from III-7, Affected
Organism Homo sapiens
Characteristics tissue: skin
source: affected skin from III-7
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with miRNeasy kit (Qiagen, Germantown, MD according to the manufacturer's instructions. The quality and quantity of the RNA samples were evaluated by NanoDrop ND-1000 Spectrophotometer (Wilmington, DE), and Agilent Bioanalyzer 2100 (Santa Clara, CA).
Label Cy3
Label protocol Total RNA was reverse transcribed and followed by generation of cDNAs. The resulting cDNAs were transcribed and labeled with cyanine 3-CTP.
Hybridization protocol The labeled antisense cRNAs were hybridized to microarrays at 65°C for 17h.
Scan protocol The arrays were washed and scanned using an Agilent scanner.
Description affected skin from III-7
Data processing The scanned images of the arrays were converted and imported into the Institute for Systems Biology’s Systems Biology Experiment Analysis System (SBEAMS) for array data normalization and further analyses.
Submission date Dec 23, 2013
Last update date Dec 24, 2013
Contact name Ji-Hoon Cho
Organization name Institute for Systems Biology
Street address 401 Terry Avenue North
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
Platform ID GPL17077
Series (2)
GSE53613 Gene expression signatures in the skin of focal palmoplantar keratoderma (FPPK) patients
GSE53616 Gene expression signatures in the skin of focal palmoplantar keratoderma (FPPK) patients, and cell lines transfected with mutant TRPV3

Data table header descriptions
VALUE Quantile normalized and log2-transformed gene expression values.

Data table
GE_BrightCorner 15.04026496
DarkCorner 5.630540488
A_23_P117082 11.61574081
A_33_P3246448 7.71596199
A_33_P3318220 5.731319031
A_33_P3236322 6.076815597
A_33_P3319925 6.693486957
A_21_P0000509 13.50286287
A_21_P0000744 8.218260499
A_24_P215804 6.240791332
A_23_P110167 12.1450508
A_33_P3211513 8.746094384
A_23_P103349 5.889323326
A_32_P61480 5.640244936
A_33_P3788124 5.951284715
A_33_P3414202 8.085472459
A_33_P3316686 7.745674324
A_33_P3300975 7.611946942
A_33_P3263061 11.99951562
A_33_P3261373 5.686500527

Total number of rows: 50739

Table truncated, full table size 1259 Kbytes.

Supplementary file Size Download File type/resource
GSM1296888_III-7_Affected_Skin.txt.gz 12.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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