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Sample GSM1287756 Query DataSets for GSM1287756
Status Public on Mar 31, 2014
Title GS-2_IFNb300_6h_rep1
Sample type RNA
Source name GS-2_IFNb300_6h_rep1
Organism Homo sapiens
Characteristics tissue: glioma
cell line: GS-2
Treatment protocol IFN-beta was dissolved in ddH2O. Cells were exposed to either IFN 300 IU/ml or ddH2O for either 6 h or 24 h before assembly of the cell pellets.
Growth protocol LNT-229 cells were grown in DMEM with 10% FCS and glutamine; GS-cells were grown in Neurobasal medium supplemented with growth factors and B27.
Extracted molecule total RNA
Extraction protocol RNA was extracted with the miRNeasy Mini Kit (Qiagen, Hilden, Germany, Cat. No. 217004); RNA qualitiy was checked with the 2100 BioAnalyzer (Agilent, Santa Clara, USA)
Label Biotin
Label protocol Sample preparation was done with 100 ng total-RNA using the GeneChip® 3´ IVT Express Kit (PN 901229) from Affymetrix (Santa Clara, USA) according to the instructions of the Affymetrix GeneChip® 3´ IVT Express Technical Manual (PN 702646). 10 µg of the fragmented cRNA were hybridized on the Gene Chip® Human Genome U133A 2.0 Arrays (Affymetrix, PN 900469)
Hybridization protocol Samples were hybridized for 16h at 45°C using Hybridization Kit materials (PN 900720, Affymetrix) according to the GeneChip® 3’ IVT Express Kit Protocol (PN 702646 Rev. 6, Affymetrix)
Scan protocol Affymetrix Gene Chip Scanner 3000 7G; AGCC
Data processing Quantile normalization / RMA summarization (GeneSpring 12.1, Agilent)
Submission date Dec 11, 2013
Last update date Mar 31, 2014
Contact name Karl Koehrer
Organization name University of Duesseldorf
Department BMFZ
Street address Universitaetsstr. 1
City Duesseldorf
ZIP/Postal code 40225
Country Germany
Platform ID GPL571
Series (1)
GSE53213 Expression data from glioma cells exposed to interferon (IFN)-beta

Data table header descriptions
VALUE normalized RMA signal, log2

Data table
AFFX-BioB-5_at 0.07142782
AFFX-BioB-M_at 0.04167843
AFFX-BioB-3_at 2.73E-04
AFFX-BioC-5_at 0.040308
AFFX-BioC-3_at 0.016111374
AFFX-BioDn-5_at 0.07781315
AFFX-BioDn-3_at 0.06959343
AFFX-CreX-5_at 0.11743641
AFFX-CreX-3_at 0.114748
AFFX-DapX-5_at 0.3335328
AFFX-DapX-M_at 0.22969437
AFFX-DapX-3_at 0.2515068
AFFX-LysX-5_at 0.040786266
AFFX-LysX-M_at 0.04144764
AFFX-LysX-3_at 0.08051157
AFFX-PheX-5_at 0.13893557
AFFX-PheX-M_at 0.10821152
AFFX-PheX-3_at 0.10789013
AFFX-ThrX-5_at 0.31895828
AFFX-ThrX-M_at 0.30834866

Total number of rows: 22277

Table truncated, full table size 489 Kbytes.

Supplementary file Size Download File type/resource
GSM1287756_7_GS-2_IFNb300_6h.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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