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Sample GSM128646 Query DataSets for GSM128646
Status Public on Oct 01, 2006
Title H157 siDJ-1 #1
Sample type RNA
Source name H157 siDJ-1 #1
Organism Homo sapiens
Characteristics NSCLC cell line H157. Transfected with siRNA oligomers targeting DJ-1 - #1
Treatment protocol H157 cells grown in RPMI w/ 10% FCS transfected 3 consecutive days annd GITC lysed 96 hours after first transfection. RNA isolated by column (Promega). Then cRNA made and labeled, hybridized to Affy U133A array
Extracted molecule total RNA
Extraction protocol Promega SV Total RNA; DNAse I treated
Label biotin
Label protocol Biotinylated cRNA were prepared using BioArray High Yield RNA Transcript Kit
Hybridization protocol Following fragmentation, 10 microg of cRNA was hybridizd onto arrays for 16 hours at 45oC in the GeneChip Hybridization Oven 640
Scan protocol Arrays were scanned with the Hewlett Packard GeneArray Scanner
Description Gene expression data from H157 cells transfected siRNA oligomers targeting DJ-1
Data processing Affymetrix GeneChip Microarray Suite 5.0 software was used for washing, scanning, and basic analysis. Sample quality was assessed by examination of 3’ to 5’ intensity ratios of certain genes.
Submission date Aug 15, 2006
Last update date Sep 19, 2006
Contact name Casey M Clements
Organization name UNC Chapel Hill
Department Lineberger Cancer Center
Lab Jenny Ting's Lab
Street address CB#7295; LCCC 11-117
City Chapel Hill
State/province NC
ZIP/Postal code 27599-7295
Country USA
Platform ID GPL96
Series (1)
GSE5519 Expression data from H157 non-small cell lung carcinoma cells with and without DJ-1 expression knocked-down

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 251.1 P 0.006187
AFFX-BioB-M_at 300.2 P 0.00039
AFFX-BioB-3_at 208.6 P 0.003212
AFFX-BioC-5_at 643.3 P 0.000446
AFFX-BioC-3_at 454.9 P 0.000509
AFFX-BioDn-5_at 599.4 P 0.000147
AFFX-BioDn-3_at 3945.6 P 0.000446
AFFX-CreX-5_at 6717.8 P 0.000044
AFFX-CreX-3_at 9495 P 0.000044
AFFX-DapX-5_at 27.2 A 0.327079
AFFX-DapX-M_at 50.5 A 0.116113
AFFX-DapX-3_at 3.7 A 0.953518
AFFX-LysX-5_at 7.5 A 0.645547
AFFX-LysX-M_at 39.2 A 0.52976
AFFX-LysX-3_at 28.5 A 0.39692
AFFX-PheX-5_at 4.9 A 0.910522
AFFX-PheX-M_at 6.1 A 0.834139
AFFX-PheX-3_at 62.4 A 0.41138
AFFX-ThrX-5_at 13 A 0.749204
AFFX-ThrX-M_at 44.8 A 0.354453

Total number of rows: 22283

Table truncated, full table size 593 Kbytes.

Supplementary data files not provided

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