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Series GSE5519 Query DataSets for GSE5519
Status Public on Oct 01, 2006
Title Expression data from H157 non-small cell lung carcinoma cells with and without DJ-1 expression knocked-down
Organism Homo sapiens
Experiment type Expression profiling by array
Summary DJ-1 expression protects H157 cells from cell death, using siRNA oligomers we knocked down DJ-1 and evaulated gene expression using Affymetrix U133A GeneChip arrays.
Keywords: siRNA comparison
Overall design H157 cells were transfected every 24 hours with siRNA oligomers for 3 consecutive days, lysed 96 hours after the first transfection, and total RNA extracted. cRNA was generated and used to probe Affymetrix U133A GeneChip microarrays.
Contributor(s) Clements CM, McNally RS, Conti BJ, Mak TW, Ting JP
Citation(s) 17015834
Submission date Aug 15, 2006
Last update date Aug 10, 2018
Contact name Casey M Clements
Organization name UNC Chapel Hill
Department Lineberger Cancer Center
Lab Jenny Ting's Lab
Street address CB#7295; LCCC 11-117
City Chapel Hill
State/province NC
ZIP/Postal code 27599-7295
Country USA
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (5)
GSM128643 H157 siMOCK
GSM128644 H157 siCTL1
GSM128645 H157 siCTL2
BioProject PRJNA96171

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