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Sample GSM128645 Query DataSets for GSM128645
Status Public on Oct 01, 2006
Title H157 siCTL2
Sample type RNA
Source name H157 siCTL2
Organism Homo sapiens
Characteristics NSCLC cell line H157. Transfected with scrambeled control non-targetting oligomer siRNA - #2
Treatment protocol H157 cells grown in RPMI w/ 10% FCS transfected 3 consecutive days annd GITC lysed 96 hours after first transfection. RNA isolated by column (Promega). Then cRNA made and labeled, hybridized to Affy U133A array
Extracted molecule total RNA
Extraction protocol Promega SV Total RNA; DNAse I treated
Label biotin
Label protocol Biotinylated cRNA were prepared using BioArray High Yield RNA Transcript Kit
Hybridization protocol Following fragmentation, 10 microg of cRNA was hybridizd onto arrays for 16 hours at 45oC in the GeneChip Hybridization Oven 640
Scan protocol Arrays were scanned with the Hewlett Packard GeneArray Scanner
Description Gene expression data from H157 cells transfected with non-targetting scrambeled control siRNA oligomers
Data processing Affymetrix GeneChip Microarray Suite 5.0 software was used for washing, scanning, and basic analysis. Sample quality was assessed by examination of 3’ to 5’ intensity ratios of certain genes.
Submission date Aug 15, 2006
Last update date Sep 19, 2006
Contact name Casey M Clements
Organization name UNC Chapel Hill
Department Lineberger Cancer Center
Lab Jenny Ting's Lab
Street address CB#7295; LCCC 11-117
City Chapel Hill
State/province NC
ZIP/Postal code 27599-7295
Country USA
Platform ID GPL96
Series (1)
GSE5519 Expression data from H157 non-small cell lung carcinoma cells with and without DJ-1 expression knocked-down

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 437.3 P 0.010317
AFFX-BioB-M_at 614.9 P 0.000147
AFFX-BioB-3_at 325 P 0.000581
AFFX-BioC-5_at 974.4 P 0.000169
AFFX-BioC-3_at 860.2 P 0.000147
AFFX-BioDn-5_at 853.8 P 0.00011
AFFX-BioDn-3_at 5306.8 P 0.000169
AFFX-CreX-5_at 10256.9 P 0.000044
AFFX-CreX-3_at 15666.3 P 0.000044
AFFX-DapX-5_at 46.9 A 0.147939
AFFX-DapX-M_at 47.3 A 0.102165
AFFX-DapX-3_at 19.5 A 0.724854
AFFX-LysX-5_at 27.5 A 0.287743
AFFX-LysX-M_at 94.6 A 0.39692
AFFX-LysX-3_at 6 A 0.559354
AFFX-PheX-5_at 4.9 A 0.941556
AFFX-PheX-M_at 6.7 A 0.772364
AFFX-PheX-3_at 93.6 A 0.327079
AFFX-ThrX-5_at 10.4 A 0.804734
AFFX-ThrX-M_at 17.1 A 0.58862

Total number of rows: 22283

Table truncated, full table size 592 Kbytes.

Supplementary data files not provided

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