|
| Status |
Public on Dec 10, 2013 |
| Title |
chick forebrains, 16h DAPT |
| Sample type |
RNA |
| |
|
| Source name |
chick forebrains, 16h DAPT
|
| Organism |
Gallus gallus |
| Characteristics |
tissue: forebrain
age: embryo HH13
|
| Treatment protocol |
Chick embryonic anterior head was dissected after 16hours roller culture with DMSP or DAPT.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was immediately extracted. The RNA quality had been controlled by 2100 Bioanalyser.
|
| Label |
Cy3
|
| Label protocol |
Total RNA (50µg) from each sample was reverse transcripted and cRNA were prepared according to the Agilent recommendations and following the one color protocol to be labelled with Cy3-CTP.
|
| |
|
| Hybridization protocol |
Hybridization was accomplished on the agilent 4x44K whole chicken genome in situ oligonucleotide microarray.
|
| Scan protocol |
Image acquisition was performed using the Agilent Scanner (Biogenouest, Rennes).
|
| Description |
Gene expression after 16hr in culture medium with DAPT
|
| Data processing |
Signals were extracted by Agilent Feature Extraction software. Data normalization was performed by a per-chip 50th percentile method by the GeneSpring Agilent GX12.
|
| |
|
| Submission date |
Dec 09, 2013 |
| Last update date |
Dec 10, 2013 |
| Contact name |
Valérie Dupé |
| E-mail(s) |
valerie.dupe@univ-rennes1.fr
|
| Organization name |
UMR6290
|
| Street address |
2 av leon Bernard
|
| City |
Rennes |
| ZIP/Postal code |
35000 |
| Country |
France |
| |
|
| Platform ID |
GPL15357 |
| Series (1) |
| GSE53129 |
Transcriptomic analysis of chic embryos forebrain lacking Notch signalling |
|
