|
| Status |
Public on May 04, 2015 |
| Title |
Bos taurus_neck muscle_D31_D0 |
| Sample type |
RNA |
| |
|
| Source name |
Bovine neck muscle collected at the slaughterhouse
|
| Organism |
Bos taurus |
| Characteristics |
tissue: neck muscle
treatment: DEXAMETHAZONE+ESTRADIOL
collection day: D0
|
| Treatment protocol |
DE: Dexamethazone 0.4 mg/animal day per os for 31 days plus estradiol 5 mg/animal/week im for 6 times
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extracted using the QiAgen RNeasy Mini Kit protocol for tissue samples
|
| Label |
Cyanine 3
|
| Label protocol |
Amplified and labeled according to Agilent One-Color Microarray-Based Gene Expression Analysis protocol
|
| |
|
| Hybridization protocol |
Hybridized according to Agilent One-Color Microarray-Based Gene Expression Analysis protocol
|
| Scan protocol |
Array was scanned according to standard Agilent protocol at a resolution of 5 microns modifying default setting to scan the slide twice at two different sensitivity settings (XDRHi 100% and XDRLo 10%)
|
| Description |
Sample of bovine neck muscle stored under vacuum at 4°C and collected at D14
|
| Data processing |
Data processed with FeatureExtraction v9.5 and normalized using cyclic lowess by R 2.11.1 statistical software
|
| |
|
| Submission date |
Dec 02, 2013 |
| Last update date |
May 04, 2015 |
| Contact name |
sara pegolo |
| E-mail(s) |
sara.pegolo@unipd.it
|
| Organization name |
university of padua
|
| Street address |
viale dell'università 16
|
| City |
legnaro |
| ZIP/Postal code |
35020 |
| Country |
Italy |
| |
|
| Platform ID |
GPL7053 |
| Series (1) |
| GSE52883 |
Comparison of gene expression profile of muscle samples stored under vacuum at +4°C up to 14 days from growth promoters-treated and control cows. |
|
