|
| Status |
Public on Nov 20, 2013 |
| Title |
ZR-75-1 Cells with shEI24 #2-1 |
| Sample type |
RNA |
| |
|
| Source name |
ZR-75-1 with shEI24
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: shEI24
cell line: ZR-75-1 Breast Cancer Cells
|
| Treatment protocol |
No Chemicalls were treated
|
| Growth protocol |
Cells were grown in RPMI 1640 with 10% FBS, 5% penicillin/streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using an RNeasy Mini Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 300 ng total RNA (GeneChip® WT Terminal Labeling and Controls Kit P/N 901525 1, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, fragmented cDNA were hybridized for 16 hr at 45C on Affymetrix GeneChip® Human Gene 1.0 ST Array. The GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Data processing |
CEL files were processed using Affymetrix GCOS software and normalized with RMA method. RMA files were imported into GeneSpring 7.3 software (Agilent Technologies) and further analyzed.
|
| |
|
| Submission date |
Nov 19, 2013 |
| Last update date |
Nov 20, 2013 |
| Contact name |
Han-Woong Lee |
| E-mail(s) |
hwl@yonsei.ac.kr
|
| Phone |
82-2-2123-7642
|
| Organization name |
Yonsei University
|
| Department |
Biochemistry
|
| Lab |
Mouse Molecular Genetics Lab
|
| Street address |
Yonsei university, Yonseiro 50
|
| City |
Seoul |
| ZIP/Postal code |
120-749 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE52508 |
Knockdown of EI24 in ZR-75-1 cells |
|
