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Status |
Public on Nov 20, 2013 |
Title |
ZR-75-1 Cells with shEI24 #1-1 |
Sample type |
RNA |
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Source name |
ZR-75-1 with shEI24
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Organism |
Homo sapiens |
Characteristics |
treatment: shEI24 cell line: ZR-75-1 Breast Cancer Cells
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Treatment protocol |
No Chemicalls were treated
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Growth protocol |
Cells were grown in RPMI 1640 with 10% FBS, 5% penicillin/streptomycin
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using an RNeasy Mini Kit (Qiagen).
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Label |
biotin
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Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 300 ng total RNA (GeneChip® WT Terminal Labeling and Controls Kit P/N 901525 1, Affymetrix).
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Hybridization protocol |
Following fragmentation, fragmented cDNA were hybridized for 16 hr at 45C on Affymetrix GeneChip® Human Gene 1.0 ST Array. The GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
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Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
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Data processing |
CEL files were processed using Affymetrix GCOS software and normalized with RMA method. RMA files were imported into GeneSpring 7.3 software (Agilent Technologies) and further analyzed.
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Submission date |
Nov 19, 2013 |
Last update date |
Nov 20, 2013 |
Contact name |
Han-Woong Lee |
E-mail(s) |
hwl@yonsei.ac.kr
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Phone |
82-2-2123-7642
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Organization name |
Yonsei University
|
Department |
Biochemistry
|
Lab |
Mouse Molecular Genetics Lab
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Street address |
Yonsei university, Yonseiro 50
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City |
Seoul |
ZIP/Postal code |
120-749 |
Country |
South Korea |
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Platform ID |
GPL6244 |
Series (1) |
GSE52508 |
Knockdown of EI24 in ZR-75-1 cells |
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