|
| Status |
Public on Dec 31, 2015 |
| Title |
NOA POST 10 (exon) |
| Sample type |
RNA |
| |
|
| Source name |
testicular biopsy with nonobstructive azoospermia
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: male
tissue: testis
testis: left
age: 28
histopatological desription: postmeiotic arrest
response to the hormonal therapy: positive response (sperm cells appered in ejaculate
|
| Treatment protocol |
Not applicable, samples extracted from untreated patients with various stages of azoospermia
|
| Growth protocol |
Not applicable, total RNA extracted from testicular biopsy cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tri-Reagent (Sigma-Aldrich, St. Louis, USA) and post-purification using RNeasy Plus Mini Kit (Qiagen, Hilden, Germany)
Testicular biopsy
|
| Label |
biotin
|
| Label protocol |
The sense-strand cDNA was fragmentized and labeled using the Affymetrix GeneChip® WT Terminal Labeling Kit (Affymetrix, Santa Clara, CA).
|
| |
|
| Hybridization protocol |
Array hybridization, washing, and scanning were performed according to manufacturer’s recommendations by using GeneChip® Hybridization, Wash and Stain Kit (Affymetrix, Santa Clara, CA).
|
| Scan protocol |
Microarrays scanned using Affymetrix 3000 Scanner according to the instructions of the manufacturer
|
| Description |
Biomarkers project
|
| Data processing |
Raw data were normalized with RMA method using Affymetrix software delivered to the scanner.
|
| |
|
| Submission date |
Sep 23, 2013 |
| Last update date |
Dec 31, 2015 |
| Contact name |
Tomasz Stokowy |
| E-mail(s) |
tomasz.stokowy@uib.no
|
| Organization name |
University of Bergen
|
| Department |
IT Division
|
| Lab |
Scientific Computing
|
| Street address |
Nygardsgaten 5
|
| City |
Bergen |
| ZIP/Postal code |
5020 |
| Country |
Norway |
| |
|
| Platform ID |
GPL10739 |
| Series (1) |
| GSE51111 |
Potential biomarkers tah could help to determine the prognosis of azoospermia treatment |
|
| Relations |
| Reanalysis of |
GSM1118254 |
