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Sample GSM1238981 Query DataSets for GSM1238981
Status Public on Dec 31, 2015
Title NOA POST 10 (exon)
Sample type RNA
Source name testicular biopsy with nonobstructive azoospermia
Organism Homo sapiens
Characteristics Sex: male
tissue: testis
testis: left
age: 28
histopatological desription: postmeiotic arrest
response to the hormonal therapy: positive response (sperm cells appered in ejaculate
Treatment protocol Not applicable, samples extracted from untreated patients with various stages of azoospermia
Growth protocol Not applicable, total RNA extracted from testicular biopsy cells
Extracted molecule total RNA
Extraction protocol Tri-Reagent (Sigma-Aldrich, St. Louis, USA) and post-purification using RNeasy Plus Mini Kit (Qiagen, Hilden, Germany)
Testicular biopsy
Label biotin
Label protocol The sense-strand cDNA was fragmentized and labeled using the Affymetrix GeneChip® WT Terminal Labeling Kit (Affymetrix, Santa Clara, CA).
Hybridization protocol Array hybridization, washing, and scanning were performed according to manufacturer’s recommendations by using GeneChip® Hybridization, Wash and Stain Kit (Affymetrix, Santa Clara, CA).
Scan protocol Microarrays scanned using Affymetrix 3000 Scanner according to the instructions of the manufacturer
Description Biomarkers project
Data processing Raw data were normalized with RMA method using Affymetrix software delivered to the scanner.
Submission date Sep 23, 2013
Last update date Dec 31, 2015
Contact name Tomasz Stokowy
Organization name University of Bergen
Department IT Division
Lab Scientific Computing
Street address Nygardsgaten 5
City Bergen
ZIP/Postal code 5020
Country Norway
Platform ID GPL10739
Series (1)
GSE51111 Potential biomarkers tah could help to determine the prognosis of azoospermia treatment
Reanalysis of GSM1118254

Data table header descriptions
VALUE RMA normalized

Data table
7896737 5.380612
7896739 2.612836
7896741 2.706971
7896743 9.82317
7896745 8.078188
7896747 9.192473
7896749 7.118347
7896751 5.599244
7896753 9.773484
7896755 7.103888
7896757 3.674902
7896758 4.892675
7896760 5.471988
7896762 9.49367
7896763 4.077932
7896764 5.128181
7896765 3.109693
7896766 5.028328
7896767 2.974311
7896768 8.790796

Total number of rows: 257430

Table truncated, full table size 4244 Kbytes.

Supplementary file Size Download File type/resource
GSM1238981_12_L_HuGene-1_0-st-v1.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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