|
| Status |
Public on May 01, 2017 |
| Title |
Chol (-) fed_2nd |
| Sample type |
RNA |
| |
|
| Source name |
Day 4_adult_fed under cholesterol deprivation
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
strain: N2
food: fed
cholesterol: cholesterol (-)
|
| Treatment protocol |
Cholesterol deprivation were started when worms hatched. Worms were exposed to fasting for 48 hr since day 2 adult.
|
| Growth protocol |
Synchronized were grown on NGM plates until young adult and then transferred to FUDR-containig plates.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip C. elegans Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
|
| Scan protocol |
GeneChips were scanned using an Affymetrix GeneChip Scanner.
|
| Data processing |
The data were analyzed with GeneSpring 12.5 GX. Expression signals of probe sets were calculated using RMA algorithm.
|
| |
|
| Submission date |
Sep 20, 2013 |
| Last update date |
May 01, 2017 |
| Contact name |
Eisuke Nishida |
| E-mail(s) |
nishida@lif.kyoto-u.ac.jp
|
| Phone |
+81-75-753-4230
|
| Organization name |
Graduate School of Biostudies, Kyoto University
|
| Department |
Department of Cell and Developmental Biology
|
| Street address |
Kitashirakawa, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8502 |
| Country |
Japan |
| |
|
| Platform ID |
GPL200 |
| Series (1) |
| GSE51046 |
Cholesterol regulates DAF-16 nuclear localization and fasting-induced longevity in C. elegans |
|
