|
| Status |
Public on Mar 05, 2014 |
| Title |
HIV infected patient 12 |
| Sample type |
RNA |
| |
|
| Source name |
PBMC
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
sample type: PBMC
disease state: HIV only
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the modified protocol of Sigma Aldrich Tri-reagent.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using the Epicentre TargetAmp Nano-g Biotin-aRNA Labeling Kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
HIV
DAA0VD27-11_04-Mar-2009
|
| Data processing |
Gene expression levels and detection p-values were exported using Illumina's GenomeStudioV2010.1. Raw data were quantile normalized and then filtered to remove non-informative probes either expressed at background level or showing little variation among samples (maximum fold change < 1.2).
|
| |
|
| Submission date |
Sep 12, 2013 |
| Last update date |
Mar 05, 2014 |
| Contact name |
Louise C Showe |
| E-mail(s) |
lshowe@wistar.org
|
| Phone |
215-898-3791
|
| Organization name |
The Wistar Institute
|
| Lab |
Dr. Louise Showe
|
| Street address |
3601 Spruce St
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE50834 |
Gene expression analysis of PBMC from HIV and HIV/TB co-infected patients |
|
