|
| Status |
Public on Dec 22, 2016 |
| Title |
Bos taurus_biceps brachii_P30 |
| Sample type |
RNA |
| |
|
| Source name |
Bos tauru_biceps brachii_prednisolone treated
|
| Organism |
Bos taurus |
| Characteristics |
treated with: prednisolone 30 mg/head daily per os for 35 days
tissue: biceps brachii muscle
|
| Treatment protocol |
prednisolone 30 mg/head daily per os for 35 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extracted using the Invitrogen TRIzol reagent and purified according to the QiAgen RNeasy MinElute Cleanup Kit protocol for tissue samples
|
| Label |
Cyanine 3
|
| Label protocol |
Amplified and labeled according to Agilent One-Color Microarray-Based Gene Expression Analysis protocol
|
| |
|
| Hybridization protocol |
Hybridized according to Agilent One-Color Microarray-Based Gene Expression Analysis protocol
|
| Scan protocol |
Array was scanned according to standard Agilent protocol at a resolution of 5 microns modifying default setting to scan the slide twice at two different sensitivity settings (XDRHi 100% and XDRLo 10%)
|
| Description |
Bovine biceps brachii samples collected at the slaughterhouse
|
| Data processing |
Data processed with FeatureExtraction v9.5 and normalized using cyclic lowess by R 2.11.1 statistical software
|
| |
|
| Submission date |
Aug 20, 2013 |
| Last update date |
Dec 22, 2016 |
| Contact name |
sara pegolo |
| E-mail(s) |
sara.pegolo@unipd.it
|
| Organization name |
university of padua
|
| Street address |
viale dell'università 16
|
| City |
legnaro |
| ZIP/Postal code |
35020 |
| Country |
Italy |
| |
|
| Platform ID |
GPL7053 |
| Series (2) |
| GSE50036 |
Comparison of gene expression profile of biceps brachii muscle samples from prednisolone-treated and control cows |
| GSE50037 |
Comparison of gene expression profile of different tissue samples from prednisolone-treated and control cows |
|
