|
| Status |
Public on Jul 31, 2013 |
| Title |
Retina_Si 20_7days_rep2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
retina, PBS, 7 days
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
strain: C57BL/8
tissue: retina
developmental stage: adult
treatment: PBS
|
| Treatment protocol |
Retinal tissues were prepared from the enucleated eyes. Four retinal tissues were pooled into 1 test tube.
|
| Growth protocol |
We intravitreally injected PBS or nanoparticles (gold and silicate nanoparticles with the diameter of 20 and 100 nm) into the right eyes of 5-week-old male C57BL/6 mice. One week later, the mice were sacrificed and the eyes were enucleated.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
10 µg of total RNA were primed with 2 µl of 100 µM T16N2 DNA primer at 70°C for 10 min, then reversed transcribed at 42°C for 1 h in the presence of 400 U SuperScript II RTase (Invitrogen), and 100 µM each dATP, dTTP, dGTP, with 25 µM dCTP, 25 µM Cy3-label
|
| |
|
| Channel 2 |
| Source name |
retina, Si20, 7 days
|
| Organism |
Mus musculus |
| Characteristics |
treatment: 20 nM Si
gender: male
age: 5-week-old
strain: C57BL/6
tissue: retina
developmental stage: adult
|
| Treatment protocol |
Retinal tissues were prepared from the enucleated eyes. Four retinal tissues were pooled into 1 test tube.
|
| Growth protocol |
We intravitreally injected PBS or nanoparticles (gold and silicate nanoparticles with the diameter of 20 and 100 nm) into the right eyes of 5-week-old male C57BL/6 mice. One week later, the mice were sacrificed and the eyes were enucleated.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions.
|
| Label |
Cy5
|
| Label protocol |
10 µg of total RNA were primed with 2 µl of 100 µM T16N2 DNA primer at 70°C for 10 min, then reversed transcribed at 42°C for 1 h in the presence of 400 U SuperScript II RTase (Invitrogen), and 100 µM each dATP, dTTP, dGTP, with 25 µM dCTP, 25 µM Cy3-label
|
| |
|
| |
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| Scan protocol |
Scanned on an Agilent G2565AA scanner.
|
| Data processing |
Agilent Feature Extraction Software (v 9.3.2.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Jul 30, 2013 |
| Last update date |
Jul 31, 2013 |
| Contact name |
Jeong Hun Kim |
| Organization name |
Seoul National University
|
| Lab |
FARB (Fight against Angiogenesis-Related Blindness) Laboratory
|
| Street address |
101, Daehak-ro, Jongno-gu
|
| City |
Seoul |
| ZIP/Postal code |
110744 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE49371 |
Investigation of Alterations in Gene Expression in the Retina Induced by Intravitreal Injection of gold and silicate nanoparticles |
|
