|
| Status |
Public on Dec 01, 2013 |
| Title |
ChIP-seq_C/EBPα_GMP |
| Sample type |
SRA |
| |
|
| Source name |
GMP_CebpaWT
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Haematopoietic progenitor cells
strain: C57BL/6 cebpa flox/flox
gender: male
antibody: Cebpa: Santa Cruz 14-AA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Chromatin 500.000 FACS sorted LSKs or GMPs was incubated with antibodies recognizing C/EBPa. For histone modification 100.000 FACS sorted cells were used for ChIP. The antibody-bound chromatin was captured with Protein-A sepharose beads, washed, de-cross-linked and precipitated. DNA was extracted using standard phenol-chloroform extraction
Precipitated DNA were mixed with 2 ng E. Coli DNA and amplified using NEB Next ChIP-seq sample prep reagent set 1 (New England Biolabs) according to manufacturers protocol.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
All data was mapped with bowtie v. 0.12.7, using standard parameters
All data was converted into BigWig format and vizualized in the UCSC genome browser
Genome_build: mm9
Supplementary_files_format_and_content: Raw data files in fastq and processed files as bigwig
|
| |
|
| Submission date |
Jul 16, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Bo Torben Porse |
| E-mail(s) |
bo.porse@finselab.dk
|
| Phone |
+45 3545 6023
|
| Organization name |
University of Copenhagen/Rigshospitalet
|
| Department |
BRIC/Finsen Laboratory
|
| Lab |
Porse group
|
| Street address |
Ole Maaloes vej 5
|
| City |
Copenhagen |
| ZIP/Postal code |
2200 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE43007 |
C/EBPa is required for self-renewal, lineage priming and maintenance of epigenetic configurations in hematopoietic stem cells |
|
| Relations |
| BioSample |
SAMN02253110 |
| SRA |
SRX323415 |
