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Series GSE43007 Query DataSets for GSE43007
Status Public on Dec 01, 2013
Title C/EBPa is required for self-renewal, lineage priming and maintenance of epigenetic configurations in hematopoietic stem cells
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Transcription factors are key regulators of hematopoieticstem cells (HSCs) and act through their ability to bind DNA andimpact on gene transcription. Their functions are interpreted inthe complex landscape of chromatin but current knowledge on howthis is achieved is very limited. C/EBPa is an importanttranscriptional regulator of hematopoiesis, but its potentialfunctions in HSCs have remained elusive. Here we report that C/EBPaserves to protect adult HSCs from apoptosis and to maintain theirquiescent state. Consequently, deletion of Cebpa is associatedwith loss of self-renewal and HSC exhaustion. By combining geneexpression analysis with genome-wide assessment of C/EBPa bindingand epigenetic configurations, we show that C/EBPa acts tomodulate the epigenetic states of genes belonging to molecularpathways important for HSC function. Moreover, we demonstrate thatC/EBPa acts as a priming factor at the HSC level to activelypromote myeloid differentiation and counteract lymphoid lineagechoice. Taken together our results show that C/EBPa is a keyregulator of HSC biology, which influences the epigeneticlandscape of HSCs in order to balance different cell fate options.
Overall design C/EBPaplha binding was assesed in heamatopoietic stem- and progenitor cells (LSK) and in myeloid progenitor cells (GMP) using ChIP-seq
Contributor(s) Hasemann M, Lauridsen FK, Waage JE, Jakobsen JS, Frank A, Schuster MB, Rapin N, Bagger FO, Hoppe P, Schroeder T, Porse BT
Citation(s) 24415956
Submission date Dec 18, 2012
Last update date May 15, 2019
Contact name Bo Torben Porse
Phone +45 3545 6023
Organization name University of Copenhagen/Rigshospitalet
Department BRIC/Finsen Laboratory
Lab Porse group
Street address Ole Maaloes vej 5
City Copenhagen
ZIP/Postal code 2200
Country Denmark
Platforms (2)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (13)
GSM1054807 CebpaWT H3K4Me3 ChIP-seq 1
GSM1054808 CebpaWT H3K4Me3 ChIP-seq 2
GSM1054809 CebpaKO H3K4Me3 ChIP-seq 1
SRA SRP017608
BioProject PRJNA184266

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Supplementary file Size Download File type/resource
GSE43007_RAW.tar 2.5 Gb (http)(custom) TAR (of BED, BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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