|
| Status |
Public on Sep 01, 2013 |
| Title |
OVCAR3_Parental_Media_Rep2 |
| Sample type |
RNA |
| |
|
| Source name |
ovarian cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Parental
growth condition: Parental_Media
|
| Treatment protocol |
Cell pellets were collected from subconfluent cultures, frozen and stored at -80ºC prior to nucleic acid extraction.
|
| Growth protocol |
Cells were maintained at 37ºC and 5% CO2 in RPMI 1640 containing 10% (v/v) FCS, 50 U.mL-1 penicillin and 50ug.mL-1 streptomycin. 'Selected' cells were cultured in 4uM PHA-533533.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cell pellets using the mirVana RNA Isolation Kit (Life Technologies).
|
| Label |
biotin
|
| Label protocol |
Affymetrix GeneChip® WT Terminal Labelling and Hybridization was performed according to the manufacturer’s protocol
|
| |
|
| Hybridization protocol |
Hybridisation was performed according to the manufacturer’s instructions (Affymetrix, Santa Clara, CA).
|
| Scan protocol |
Arrays were washed using an Affymetrix fluidics stations and scanned using the Gene Chip Scanner 3000
|
| Data processing |
CEL files were produced using the Affymetrix® GeneChip® Command Console® Software (AGCC)
|
| |
|
| Submission date |
Jul 16, 2013 |
| Last update date |
Sep 01, 2013 |
| Contact name |
Dariush Etemadmoghadam |
| E-mail(s) |
dariush.etemadmoghadam@petermac.org
|
| Phone |
61396561149
|
| Organization name |
Peter MacCallum Cancer Centre
|
| Street address |
St Andrew's Place
|
| City |
East Melbourne |
| ZIP/Postal code |
3002 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE48919 |
Gene expression analysis of OVCAR-3 and CDK2 resistant sublines |
| GSE48921 |
Gene expression and copy number analysis of OVCAR-3 and CDK2 resistant sublines |
|
