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Sample GSM1180564 Query DataSets for GSM1180564
Status Public on Jul 04, 2013
Title Bone marrow 100 clone HIGH
Sample type RNA
Source name MSC clone after 28 days of culture, CD146High
Organism Homo sapiens
Characteristics cell type: cultivated bone marrow-derived mesenchymal stem cells
surface cd146: high
donor: 100
Treatment protocol No treatment.
Growth protocol MSC non-clonal or clonal were cultivated with aMEM (minimum essential medium) + 10% FCS (fetal calf serum) + 10µg/ml ciprofloxacin during 21 and 28 days, respectively.
Extracted molecule total RNA
Extraction protocol Use of RNeasy kit (Qiagen). RNA purity and integrity were checked by use of Bioanalyzer 2100 (Agilent).
Label biotin
Label protocol Biotinylated cRNA was amplified by the small sample labelling protocol (two cycle amplification kit, Affymetrix).
Hybridization protocol Hybridized on GeneChip HuGene 1.0 ST oligonucleotide microarrays (Affymetrix).
Scan protocol GeneChip scanner 3000 and software AGCC.
Description MoPur100 HIGH
Gene expression data from human cultivated bone marrow-derived MSC during 28 days.
Data processing Expression signal values and P-values were obtained for each probe set by use of Partek Genomics suite (Partek) by the Robust Multichip Averaging algorithm in normalization. Principal component and gene expression analysis involved use of MeV.
Submission date Jul 03, 2013
Last update date Jul 04, 2013
Contact name Nicolas Espagnolle
Organization name STROMALab, Université de Toulouse, CNRS ERL5311, EFS, INP-ENVT, Inserm U1031, UPS, Toulouse, France
Street address Bâtiment INCERE 4 bis, avenue Hubert Curien
City Toulouse
ZIP/Postal code 31100
Country France
Platform ID GPL10739
Series (1)
GSE48540 CD146 expression in mesenchymal stem cells is associated with vascular smooth muscle commitment

Data table header descriptions
VALUE GC-RMA normalized signal intensity

Data table
7892501 47.3123
7892502 41.4057
7892503 6.16748
7892504 275.668
7892505 29.4484
7892506 29.1329
7892507 14.9852
7892508 26.4322
7892509 2336.94
7892510 11.6745
7892511 10.0238
7892512 158.79
7892513 6.62904
7892514 1204.19
7892515 377.101
7892516 13.6701
7892517 20.6533
7892518 8.42293
7892519 31.7722
7892520 515.067

Total number of rows: 257430

Table truncated, full table size 4004 Kbytes.

Supplementary file Size Download File type/resource
GSM1180564_12.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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