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Sample GSM1180553 Query DataSets for GSM1180553
Status Public on Jul 04, 2013
Title Bone marrow 72 non-clonal
Sample type RNA
Source name MSC non-clonal after 21 days of culture
Organism Homo sapiens
Characteristics cell type: cultivated bone marrow-derived mesenchymal stem cells
donor: 72
Treatment protocol No treatment.
Growth protocol MSC non-clonal or clonal were cultivated with aMEM (minimum essential medium) + 10% FCS (fetal calf serum) + 10µg/ml ciprofloxacin during 21 and 28 days, respectively.
Extracted molecule total RNA
Extraction protocol Use of RNeasy kit (Qiagen). RNA purity and integrity were checked by use of Bioanalyzer 2100 (Agilent).
Label biotin
Label protocol Biotinylated cRNA was amplified by the small sample labelling protocol (two cycle amplification kit, Affymetrix).
Hybridization protocol Hybridized on GeneChip HuGene 1.0 ST oligonucleotide microarrays (Affymetrix).
Scan protocol GeneChip scanner 3000 and software AGCC.
Description MoPur72 NC
Gene expression data from human cultivated bone marrow-derived MSC during 21 days.
Data processing Expression signal values and P-values were obtained for each probe set by use of Partek Genomics suite (Partek) by the Robust Multichip Averaging algorithm in normalization. Principal component and gene expression analysis involved use of MeV.
Submission date Jul 03, 2013
Last update date Jul 04, 2013
Contact name Nicolas Espagnolle
Organization name STROMALab, Université de Toulouse, CNRS ERL5311, EFS, INP-ENVT, Inserm U1031, UPS, Toulouse, France
Street address Bâtiment INCERE 4 bis, avenue Hubert Curien
City Toulouse
ZIP/Postal code 31100
Country France
Platform ID GPL10739
Series (1)
GSE48540 CD146 expression in mesenchymal stem cells is associated with vascular smooth muscle commitment

Data table header descriptions
VALUE GC-RMA normalized signal intensity

Data table
7892501 46.1821
7892502 32.6716
7892503 6.87093
7892504 411.65
7892505 6.83492
7892506 25.3492
7892507 30.7589
7892508 14.2542
7892509 2179.41
7892510 12.0504
7892511 7.50043
7892512 361.765
7892513 5.01624
7892514 1019.93
7892515 462.235
7892516 20.0728
7892517 33.833
7892518 3.03809
7892519 28.0988
7892520 607.143

Total number of rows: 257430

Table truncated, full table size 3999 Kbytes.

Supplementary file Size Download File type/resource
GSM1180553_1.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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