|
| Status |
Public on Dec 01, 2006 |
| Title |
mutant spt15, 5% EtOH, 60 g/L glucose, replicate 2 (OUTLIER) |
| Sample type |
RNA |
| |
|
| Source name |
BY4741 (p416-Tefmut2-spt15-300)
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
genetic and environmental variation indicated in Sample_title
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Strains were cultured at 30C with 225 rpm orbital shaking in 30 mL YSC -URA medium with the indicated ethanol concentration in 50 mL closed conical tubes. The cultures harvested at approximately OD 0.4. Protocols from RAmbion RiboPure Yeast RNA extraction kits were followed.
|
| Label |
biotin
|
| Label protocol |
The extracted RNA was processed by Ambion Services using the Affymetrix protocols with the exception of Ambion ArrayScript substituted for SuperScript and Vivaspin Concentrators substituted for Qiagen cleanup columns.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
mutant spt15, 5% EtOH, 60 g/L glucose
|
| Data processing |
Bioconductor with RMA background correction, qspline normalization, and Li-Wong expression value summaries
|
| |
|
| Submission date |
Jun 28, 2006 |
| Last update date |
Dec 01, 2006 |
| Contact name |
Joel Moxley |
| E-mail(s) |
jfmoxley@mit.edu
|
| Organization name |
MIT
|
| Street address |
77 Massachusettes Ave
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE5185 |
Engineering Yeast Transcription Machinery for Improved Ethanol Tolerance and Production |
|
