 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Nov 30, 2006 |
| Title |
Engineering Yeast Transcription Machinery for Improved Ethanol Tolerance and Production |
| Platform organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
| Sample organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
Global transcription machinery engineering (gTME) is an approach for reprogramming gene transcription to elicit cellular phenotypes important for technological applications. Here we show the application of gTME to Saccharomyces cerevisiae for improved glucose/ethanol tolerance, a key trait for many biofuels programs. Mutagenesis of the transcription factor Spt15p and selection led to dominant mutations that conferred increased tolerance and more efficient glucose conversion to ethanol. The desired phenotype results from the combined effect of three separate mutations in the SPT15 gene [serine substituted for phenylalanine (Phe177Ser) and, similarly, Tyr195His, and Lys218Arg]. Thus, gTME can provide a route to complex phenotypes that are not readily accessible by traditional methods.
Keywords: stress response
|
| |
|
| Overall design |
We measured transcription levels for two strains (wild type control and mutant spt15) under normal (0% ethanol, 20 g/L glucose) and stress (5% ethanol, 60 g/L glucose) in biological triplicate.
|
| |
|
| Contributor(s) |
Alper H, Moxley J, Nevoigt E, Fink GR, Stephanopoulos G |
| Citation(s) |
17158319 |
| Submission date |
Jun 28, 2006 |
| Last update date |
Feb 21, 2017 |
| Contact name |
Joel Moxley |
| E-mail(s) |
jfmoxley@mit.edu
|
| Organization name |
MIT
|
| Street address |
77 Massachusettes Ave
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL2529 |
[Yeast_2] Affymetrix Yeast Genome 2.0 Array |
|
| Samples (12)
|
| GSM116815 |
wild type, 0% EtOH, 20 g/L glucose, replicate 1 |
| GSM116816 |
wild type, 0% EtOH, 20 g/L glucose, replicate 2 |
| GSM116817 |
wild type, 0% EtOH, 20 g/L glucose, replicate 3 |
| GSM116818 |
mutant spt15, 0% EtOH, 20 g/L glucose, replicate 1 |
| GSM116819 |
mutant spt15, 0% EtOH, 20 g/L glucose, replicate 2 |
| GSM116820 |
mutant spt15, 0% EtOH, 20 g/L glucose, replicate 3 |
| GSM116821 |
wild type, 5% EtOH, 60 g/L glucose, replicate 1 |
| GSM116822 |
wild type, 5% EtOH, 60 g/L glucose, replicate 2 |
| GSM116823 |
wild type, 5% EtOH, 60 g/L glucose, replicate 3 |
| GSM116824 |
mutant spt15, 5% EtOH, 60 g/L glucose, replicate 1 |
| GSM116825 |
mutant spt15, 5% EtOH, 60 g/L glucose, replicate 2 (OUTLIER) |
| GSM116826 |
mutant spt15, 5% EtOH, 60 g/L glucose, replicate 3 |
|
| Relations |
| BioProject |
PRJNA96489 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE5185_RAW.tar |
19.1 Mb |
(http)(custom) |
TAR (of CEL) |
|
|
|
|
 |
Less...
More...