|
| Status |
Public on Dec 31, 2014 |
| Title |
Human none marrow-derived mesenchymal stromal primary culture (hMSC_Male) |
| Sample type |
RNA |
| |
|
| Source name |
Human none marrow-derived mesenchymal stromal primary culture
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: hMSC
sample origin: Human bone marrow-derived mesenchymal stromal cell
gender: Male
|
| Growth protocol |
Primary hMSCs were obtained from the hip of a healthy donor after informed consent. Mononuclear cells from the bone marrow were isolated using standard density centrifugation and cultured in minimum essential medium (MEM) Alpha medium (cat. no. 22561) supplemented with 20% FBS (PAA Laboratories), GlutaMAX, penicillin (100U/mL), and streptomycin (100 mg/mL) (all from Life Technologies) at 37C and 5% CO2. Non-adherent cells were removed after approximately 24 h by replacement of the medium. Subsequently, the adherent cells were allowed to expand until 70% confluence (5–6 days) before they were harvested and further expanded. iMSC#3 were grown under the same condition as hMSCs, but the medium was supplemented with 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the standard TRIzol procedure (Invitrogen), and further purified with an RNeasy mini column (Qiagen GmbH, Hilden, Germany), according to the manufacturers’ instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data was quantile normalisation in GenomeStudio (Illumina)
|
| |
|
| Submission date |
Jun 05, 2013 |
| Last update date |
Dec 31, 2014 |
| Contact name |
Leonardo A. Meza-Zepeda |
| E-mail(s) |
L.A.Meza-Zepeda@ous-research.no
|
| Phone |
+47-99035706
|
| Organization name |
Oslo University Hospital
|
| Department |
Department of Tumor Biology
|
| Lab |
Meza-Zepeda
|
| Street address |
Ullernchausseen 70
|
| City |
Oslo |
| ZIP/Postal code |
0310 |
| Country |
Norway |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE47648 |
Generation and Characterization of an immortalized human mesenchymal stromal cell line model [Illumina iMSC#3 and hMSC primary cultures] |
| GSE47651 |
Generation and Characterization of an immortalized human mesenchymal stromal cell line model |
|
