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Sample GSM1154012 Query DataSets for GSM1154012
Status Public on Dec 31, 2014
Title Human none marrow-derived mesenchymal stromal primary culture (hMSC_Male)
Sample type RNA
Source name Human none marrow-derived mesenchymal stromal primary culture
Organism Homo sapiens
Characteristics cell type: hMSC
sample origin: Human bone marrow-derived mesenchymal stromal cell
gender: Male
Growth protocol Primary hMSCs were obtained from the hip of a healthy donor after informed consent. Mononuclear cells from the bone marrow were isolated using standard density centrifugation and cultured in minimum essential medium (MEM) Alpha medium (cat. no. 22561) supplemented with 20% FBS (PAA Laboratories), GlutaMAX, penicillin (100U/mL), and streptomycin (100 mg/mL) (all from Life Technologies) at 37C and 5% CO2. Non-adherent cells were removed after approximately 24 h by replacement of the medium. Subsequently, the adherent cells were allowed to expand until 70% confluence (5–6 days) before they were harvested and further expanded. iMSC#3 were grown under the same condition as hMSCs, but the medium was supplemented with 10% FBS.
Extracted molecule total RNA
Extraction protocol RNA was isolated using the standard TRIzol procedure (Invitrogen), and further purified with an RNeasy mini column (Qiagen GmbH, Hilden, Germany), according to the manufacturers’ instructions.
Label Biotin
Label protocol Biotinylated cRNA was prepared with the Ambion MessageAmp kit for Illumina arrays
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Data processing The data was quantile normalisation in GenomeStudio (Illumina)
Submission date Jun 05, 2013
Last update date Dec 31, 2014
Contact name Leonardo A. Meza-Zepeda
Phone +47-99035706
Organization name Oslo University Hospital
Department Department of Tumor Biology
Lab Meza-Zepeda
Street address Ullernchausseen 70
City Oslo
ZIP/Postal code 0310
Country Norway
Platform ID GPL10558
Series (2)
GSE47648 Generation and Characterization of an immortalized human mesenchymal stromal cell line model [Illumina iMSC#3 and hMSC primary cultures]
GSE47651 Generation and Characterization of an immortalized human mesenchymal stromal cell line model

Data table header descriptions
VALUE Quantile normalised intensities in GenomeStudio (Illumina)
hMSC_Male.Detection Pval

Data table
ID_REF VALUE hMSC_Male.Detection Pval
ILMN_1343291 22835.9900 0.0000
ILMN_1343295 8287.3640 0.0000
ILMN_1651199 56.2238 0.9364
ILMN_1651209 77.2118 0.1338
ILMN_1651210 65.0882 0.6325
ILMN_1651221 75.3813 0.1948
ILMN_1651228 4885.7610 0.0000
ILMN_1651229 220.2815 0.0000
ILMN_1651230 62.0695 0.7623
ILMN_1651232 76.8791 0.1494
ILMN_1651235 86.0714 0.0208
ILMN_1651236 58.9877 0.8571
ILMN_1651237 140.4480 0.0000
ILMN_1651238 57.1432 0.9195
ILMN_1651249 62.0876 0.7623
ILMN_1651253 74.8996 0.2078
ILMN_1651254 2501.0750 0.0000
ILMN_1651259 114.8925 0.0000
ILMN_1651260 58.1199 0.8909
ILMN_1651262 6540.9470 0.0000

Total number of rows: 47318

Table truncated, full table size 1309 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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