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Series GSE47648 Query DataSets for GSE47648
Status Public on Dec 31, 2014
Title Generation and Characterization of an immortalized human mesenchymal stromal cell line model [Illumina iMSC#3 and hMSC primary cultures]
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Human mesenchymal stromal cells (hMSCs) show great potential for clinical and experimental use. Self-renewal and multipotent differentiation capacity are key elements in the increasing interest of this cell type. However, a disadvantage of primary hMSCs is the limited in vitro lifespan, but this can be overcome by introduction of the catalytic subunit of human telomerase reverse transcriptase (TERT). In this paper, the generation and characterization of a TERT-immortalized, non-tumorigenic human bone marrow-derived stromal mesenchymal model cell line is described. The resulting cell line, iMSC#3, maintained capacity to differentiate into osteoblasts and adipocytes, and growth characteristics comparable to primary hMSCs into after long-term culturing (155 population doublings). A detailed characterization of the mRNA- and microRNA transcriptomes during adipocyte differentiation also showed that the iMSC#3 recapitulates this process at the molecular level. Furthermore, iMSC#3 showed no major differences from hMSCs regarding surface marker expression. The cell line had a normal karyotype, and high-resolution array comparative genomic hybridization confirmed that the cells in general had normal copy number. The gene expression profiles of immortalized and primary hMSCs were also similar, whereas the corresponding methylation profiles were more diverse. In conclusion, a human bone marrow-derived stromal cell line was developed, which can be used for basic studies of mesenchymal cells and to functionally study transgenes that might play a role in oncogenic transformation.
Overall design Comparison of gene expression patterns of iMSC#3 and hMSC primary cultures
Contributor(s) Skårn M, Noordhuis P, Veuger MJ, Wang MY, Kresse SH, Egeland EV, Micci F, Namløs HM, Håkelien A, Olafsrud SM, Lorenz S, Haraldsen G, Kvalheim G, Meza-Zepeda LA, Myklebost O
Citation(s) 24857590
Submission date Jun 05, 2013
Last update date Aug 16, 2019
Contact name Leonardo A. Meza-Zepeda
Phone +47-99035706
Organization name Oslo University Hospital
Department Department of Tumor Biology
Lab Meza-Zepeda
Street address Ullernchausseen 70
City Oslo
ZIP/Postal code 0310
Country Norway
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (3)
GSM1154010 Immortalized human bone marrow-derived mesenchymal stromal cell line iMSC#3 (Culture I)
GSM1154011 Human none marrow-derived mesenchymal stromal primary culture (hMSC_Female)
GSM1154012 Human none marrow-derived mesenchymal stromal primary culture (hMSC_Male)
This SubSeries is part of SuperSeries:
GSE47651 Generation and Characterization of an immortalized human mesenchymal stromal cell line model
BioProject PRJNA207292

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE47648_RAW.tar 26.2 Mb (http)(custom) TAR
GSE47648_non-normalized.txt.gz 1.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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