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| Status |
Public on Nov 27, 2006 |
| Title |
Yeast gcr1 null mutant in raffinose rep1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
yeast, 3% raffinose
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
gcr1 null mutant, S288C background, grown to early/mid log in YEP containing indicated carbon source
|
| Extracted molecule |
total RNA |
| Label |
Cy5
|
| |
|
| Channel 2 |
| Source name |
yeast, 3% raffinose
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
wild type strain, S288C background, grown to early/mid log in YEP containing indicated carbon source
|
| Extracted molecule |
total RNA |
| Label |
Cy3
|
| |
|
| |
| Description |
labeling done with Agilent's Low RNA Input Linear Amp kit; hyb and slide processing done according to Agilent's instructions
|
| Data processing |
log2 ratio after ratio-based and LOWESS normalization in Acuity 4.0
|
| |
|
| Submission date |
Jun 08, 2006 |
| Last update date |
Nov 27, 2006 |
| Contact name |
Kellie Barbara |
| E-mail(s) |
kellie.barbara@gmail.com
|
| Organization name |
Univ. of Southern MS
|
| Department |
Biological Sciences
|
| Lab |
Santangelo Lab
|
| Street address |
118 College Dr. #5018
|
| City |
Hattiesburg |
| State/province |
MS |
| ZIP/Postal code |
39406 |
| Country |
USA |
| |
|
| Platform ID |
GPL2883 |
| Series (1) |
| GSE5027 |
Transcriptome response to gcr1 deletion in raffinose, galactose, and glucose |
|
| Data table header descriptions |
| ID_REF |
|
| VALUE |
ratio-based and LOWESS normalized log2 ratio |
| F635 Mean - B635 |
channel 1 mean minus background |
| F532 Mean - B532 |
channel 2 mean minus background |
| Data table |
| ID_REF |
VALUE |
F635 Mean - B635 |
F532 Mean - B532 |
| 1 |
-8.598 |
17 |
5404 |
| 2 |
-2.421 |
5 |
28 |
| 3 |
0.55 |
282 |
218 |
| 4 |
0.688 |
150 |
101 |
| 5 |
0.041 |
161 |
171 |
| 6 |
-1.974 |
6 |
24 |
| 7 |
0.582 |
143 |
106 |
| 8 |
-1.784 |
8 |
28 |
| 9 |
0.164 |
175 |
176 |
| 10 |
-0.536 |
257 |
411 |
| 11 |
1.456 |
564 |
225 |
| 12 |
1.553 |
825 |
313 |
| 13 |
-0.873 |
20 |
39 |
| 14 |
0.385 |
3480 |
3006 |
| 15 |
-1.951 |
9 |
36 |
| 16 |
0.852 |
573 |
350 |
| 17 |
-1.208 |
19 |
51 |
| 18 |
0.4 |
1149 |
1008 |
| 19 |
-0.446 |
714 |
1061 |
| 20 |
-1.836 |
7 |
25 |
Total number of rows: 10807
Table truncated, full table size 195 Kbytes.
| Supplementary data files not provided |
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