|
| Status |
Public on Feb 28, 2014 |
| Title |
Limousin (Cy5) vs. Holstein-Friesian (Cy3) 3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
LIM 8237
|
| Organism |
Bos taurus |
| Characteristics |
gender: bull
breed: Limousine (LIM)
age: 15 months
tissue: muscle m. semitendinosus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions (A&A BIOTECHNOLOGY)
|
| Label |
Cy5
|
| Label protocol |
according to the manufacturer's protocol
|
| |
|
| Channel 2 |
| Source name |
H-F 29026
|
| Organism |
Bos taurus |
| Characteristics |
gender: bull
breed: Holstein-Friesian (H-F)
age: 15 months
tissue: muscle m. semitendinosus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions (A&A BIOTECHNOLOGY)
|
| Label |
Cy3
|
| Label protocol |
according to the manufacturer's protocol
|
| |
|
| |
| Hybridization protocol |
according to the manufacturer's protocol
|
| Scan protocol |
Scanned on an Agilent G2505C US10353831 scanner. Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1).
|
| Description |
Biological replicate 3 of 4. Control - Limousine muscle tissue
|
| Data processing |
Agilent Feature Extraction Software (v 10.7.3.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Apr 26, 2013 |
| Last update date |
Jul 07, 2015 |
| Contact name |
Tomasz Sadkowski |
| E-mail(s) |
tomasz_sadkowski@sggw.pl
|
| Phone |
+48225936263
|
| Fax |
+48228472452
|
| URL |
http://sadkowski.info
|
| Organization name |
Warsaw University of Life Sciences - SGGW
|
| Department |
Department of Physiological Sciences
|
| Street address |
Nowoursynowska 159
|
| City |
Warsaw |
| ZIP/Postal code |
02-776 |
| Country |
Poland |
| |
|
| Platform ID |
GPL7053 |
| Series (1) |
| GSE46411 |
Transcriptomic profiles of beef marbling |
|
