|
| Status |
Public on Jun 10, 2013 |
| Title |
Somatotroph adenoma 323 |
| Sample type |
RNA |
| |
|
| Source name |
Adenoma_tertile 3_SA treatment
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
age: 60
adenoma: pituitary
e-cadherin expression: high
treatment: SA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction followed by Rneasy clean up kit (qiagen) was performed according to the manufacturer´s instruction
|
| Label |
biotin
|
| Label protocol |
Double-stranded cDNA and biotin-labeled cRNA probes were made from 5 μg of total RNA by use of GeneChip® Expression 3' Amplification One-Cycle Target Labeling kit (Affymetrix).
|
| |
|
| Hybridization protocol |
The cRNA was hybridized to HG U133 plus 2.0 Arrays (Affymetrix) followed by washing and staining on the GeneChips Fluidics Station 450 (Affymetrix)
|
| Scan protocol |
Signal intensities were detected by Affymetrix GeneChip Scanner 3000
|
| Data processing |
The scanned images were processed using GCOS 1.4 (Affymetrix) according to the manufacturer. The resulting data sets (CEL files) were imported into ArrayAssist Expression Software (v5.2.0;Iobion Informatics LLC) and normalized using the PLIER algorithm in Array Assist to calculate relative signal values for each probe set.
|
| |
|
| Submission date |
Apr 23, 2013 |
| Last update date |
Jun 10, 2013 |
| Contact name |
Tove Lekva |
| E-mail(s) |
tove.lekva@medisin.uio.no, tove.lekva@ous-hf.no
|
| Organization name |
OUS-HF, Rikshospitalet
|
| Department |
Section of Specialized Endocrinology and Institute for Internal Medicine
|
| Street address |
Sognsvannsveien 20
|
| City |
Oslo |
| ZIP/Postal code |
0027 |
| Country |
Norway |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE46311 |
Gene expression analysis of growth hormone producing somatotroph pituitary adenomas |
|
