|
| Status |
Public on Jul 01, 2013 |
| Title |
macrophages control (IFNAR wt/ko) #1 |
| Sample type |
SRA |
| |
|
| Source name |
mouse peritoneal macrophages
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: 18 weeks
gender: male
cell type: peritoneal macrophage
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were lysed immediately in µMACS™ mRNA Isolation Kit lysis buffer and cleaned with with LysateClear Columns (Miltenyi Biotec). mRNA was enriched using Oligo(dT) beads.
Poly-dT-enrichment, DNAse treatment, chemical fragmentation, ss-Synthesis, strand specific library prep according to: Parkhomchuk D, Borodina T, Amstislavskiy V, Banaru M, Hallen L, Krobitsch S, Lehrach H, Soldatov A. Transcriptome analysis by strand-specific sequencing of complementary DNA. Nucleic Acids Res. 2009 Jul 20. doi:10.1093/nar/gkp596
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
S1895_mp_Kontrolle_1
Strand specific RNA-Seq
|
| Data processing |
Read Alignment: pBWA version 0.5.9-r32-MPI-patch2 against mm9 (UCSC)
Counts per feature: Reads per exon were computed using bedtools version 2.11, summarized per gene based on Ensembl Gene 61 annotation.
Normalization of reads per gene: The raw counts (absolute number of reads) were normalized with the DESeq R package (v.1.8.1) and sample to sample correlation (Pearson's correlation coefficient) was computed based on the normalized counts.
Genome_build: mm9
Supplementary_files_format_and_content: Tab-delimited 'counts' file with sample size normalized counts per gene (rows) and sample (columns)
|
| |
|
| Submission date |
Mar 20, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Tina Schumann |
| E-mail(s) |
Tina.Schumann@tu-dresden.de
|
| Organization name |
Institute of Immunology
|
| Department |
Medical Faculty "Carl Gustav Carus"
|
| Street address |
Fetscherstraße 74
|
| City |
Dresden |
| ZIP/Postal code |
01307 |
| Country |
Germany |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE45358 |
Gene expression analysis of murine peritoneal macrophages deficient for SAMHD1 and IFNAR |
|
| Relations |
| SRA |
SRX253175 |
| BioSample |
SAMN01985470 |
