|
| Status |
Public on Mar 27, 2013 |
| Title |
Mouse Wild Type 14.5 dpp small RNA |
| Sample type |
SRA |
| |
|
| Source name |
testes
|
| Organism |
Mus musculus |
| Characteristics |
tissue: testes
developmental stage: 14.5-dpp
strain: C57BL/6J
genotype: wild-type
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Small RNA libraries were constructed and sequenced as described (Ghildiyal et al., 2008; Seitz et al., 2008) except that 18–35 nt RNA was isolated and 2S rRNA depletion omitted.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
small RNA
|
| Data processing |
genome build: mm9
Small RNA sequence analysis was as described (Li et al., 2009)
Supplementary_files_format_and_content: BED file containing annotations of piRNA-producing genes and csv file containing rpkm values available on Series record
|
| |
|
| Submission date |
Mar 12, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Christian Roy |
| Organization name |
Umass Med
|
| Street address |
364 Plantation St
|
| City |
Worcester |
| State/province |
Massachusetts |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platform ID |
GPL13112 |
| Series (2) |
| GSE44690 |
An Ancient Transcription Factor Initiates the Burst of piRNA Production During Early Meiosis in Mouse Testes |
| GSE45049 |
An Ancient Transcription Factor Initiates the Burst of piRNA Production During Early Meiosis in Mouse Testes (smallRNA-Seq) |
|
| Relations |
| SRA |
SRX248864 |
| BioSample |
SAMN01975524 |
