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Series GSE45049 Query DataSets for GSE45049
Status Public on Mar 27, 2013
Title An Ancient Transcription Factor Initiates the Burst of piRNA Production During Early Meiosis in Mouse Testes (smallRNA-Seq)
Organisms Gallus gallus; Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Animal germ cells produce PIWI-interacting RNAs (piRNAs), small silencing RNAs that suppress transposons and enable gamete maturation. Mammalian transposon-silencing piRNAs accumulate early in spermatogenesis, whereas pachytene piRNAs are produced later during post-natal spermatogenesis and account for >95% of all piRNAs in the adult mouse testis. Mutants defective for pachytene piRNA pathway proteins fail to produce mature sperm, but neither the piRNA precursor transcripts nor the trigger for pachytene piRNA production is known. Here, we show that the transcription factor A-MYB initiates pachytene piRNA production. A-MYB drives transcription of both pachytene piRNA precursor RNAs and the mRNAs for core piRNA biogenesis factors, including MIWI, the protein through which pachytene piRNAs function. A-MYB regulation of piRNA pathway proteins and piRNA genes creates a coherent feed-forward loop that ensures the robust accumulation of pachytene piRNAs. This regulatory circuit, which can be detected in rooster testes, likely predates the divergence of birds and mammals.
 
Overall design smallRNA-Seq in mouse and rooster testes
 
Contributor(s) Li XZ, Roy CK, Zamore PD
Citation(s) 23523368
Submission date Mar 12, 2013
Last update date May 15, 2019
Contact name Christian Roy
Organization name Umass Med
Street address 364 Plantation St
City Worcester
State/province Massachusetts
ZIP/Postal code 01605
Country USA
 
Platforms (4)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
GPL9385 Illumina Genome Analyzer II (Gallus gallus)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (32)
GSM1096582 Mouse Wild Type 10.5 dpp small RNA
GSM1096583 Mouse Wild Type 12.5 dpp small RNA
GSM1096584 Mouse Wild Type 14.5 dpp small RNA
This SubSeries is part of SuperSeries:
GSE44690 An Ancient Transcription Factor Initiates the Burst of piRNA Production During Early Meiosis in Mouse Testes
Relations
SRA SRP019258
BioProject PRJNA194540

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE45049_102-assembled.piRNA.transcripts.bed.gz 19.6 Kb (ftp)(http) BED
GSE45049_smallRNA.rpkm.matrix.csv.gz 56.4 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record
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